{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T11:42:01Z","timestamp":1791891721528,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2004,9,9]],"date-time":"2004-09-09T00:00:00Z","timestamp":1094688000000},"content-version":"vor","delay-in-days":8,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2004,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Blood cultures and cultures of disc material are required to identify and treat bacterial agents responsible for septic spondylodiscitis, but these methods have limited sensitivities. We undertook this study to compare nonculture amplification\u2010based DNA analysis with conventional culture of disc aspirate.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Nineteen patients with spondylodiscitis, including 11 with a history of spinal surgery, presented with negative blood cultures and underwent percutaneous disc or epidural abscess puncture for bacterial diagnosis. Amplification by polymerase chain reaction was performed on 16S ribosomal DNA universal target genes and <jats:italic>femA<\/jats:italic> staphylococci\u2010specific target genes in all patients, and on the <jats:italic>upstream p34<\/jats:italic> mycobacterial gene in 1 patient. Species identification relied on amplicon sequencing and comparison with templates from GenBank. Amplification of the <jats:italic>femA<\/jats:italic> gene led to subsequent testing for methicillin resistance by amplification of the <jats:italic>mecA<\/jats:italic> gene. Further assessment using a staphylococci\u2010 and methicillin resistance\u2013specific DNA array was performed on 3 samples.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Microbiologic and molecular assays identified the causative organism in 14 of 19 patients (74%) and 19 of 19 patients (100%), respectively. In culture\u2010positive patients, DNA\u2010based and microbiologic results were highly correlated. Five agents (<jats:italic>Staphylococcus simulans, Staphylococcus sciuri, Brucella<\/jats:italic> species, <jats:italic>Actinomyces israelii<\/jats:italic>, and <jats:italic>Mycobacterium tuberculosis<\/jats:italic> complex) were identified only by DNA\u2010based methods. In 1 sample, <jats:italic>Corynebacterium jeikeium<\/jats:italic> and coagulase\u2010negative <jats:italic>Staphylococcus<\/jats:italic> were both cultured, whereas DNA analysis identified only <jats:italic>Staphylococcus hominis<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>DNA\u2010based methods are highly sensitive and specific. They can usefully complement standard microbiologic methods for identifying the cause of infectious spondylodiscitis and contribute to species\u2010specific therapeutic orientation in patients with negative blood and disc aspirate cultures.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.20462","type":"journal-article","created":{"date-parts":[[2004,9,14]],"date-time":"2004-09-14T08:47:59Z","timestamp":1095151679000},"page":"2985-2994","source":"Crossref","is-referenced-by-count":74,"title":["The etiologic diagnosis of infectious discitis is improved by amplification\u2010based DNA analysis"],"prefix":"10.1002","volume":"50","author":[{"given":"Fr\u00e9d\u00e9ric","family":"Lecouvet","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonid","family":"Irenge","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Vandercam","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adrien","family":"Nzeusseu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sandrine","family":"Hamels","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Luc","family":"Gala","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,9,9]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1093\/clinids\/19.4.746"},{"key":"e_1_2_6_3_2","first-page":"401","article-title":"Infectious spondylodiscitis. 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