{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T16:47:54Z","timestamp":1791478074984,"version":"4.3.4"},"reference-count":15,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,11,4]],"date-time":"2004-11-04T00:00:00Z","timestamp":1099526400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Japanese Ministry of Health, Labor, and Welfare"},{"DOI":"10.13039\/501100001700","name":"Japanese Ministry of Education, Science, Sports and Culture","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To detect and characterize the autoreactive CD8+ T cells to major histocompatibility complex class I chain\u2013related gene A (MICA), a stress\u2010inducible antigen preferentially expressed on the epithelium and endothelium, in patients with Beh\u00e7et's disease (BD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A candidate for the antigenic MICA peptide was selected based on its predicted binding affinity for HLA\u2013B51 and proteasomal cleavage sites. Peripheral blood T cells from 14 patients with BD and 15 healthy controls were repeatedly stimulated with the MICA peptide, and the specific T cell response was measured by peptide\u2010induced interferon\u2010\u03b3. Cytotoxic T lymphocyte activity was examined by chromium\u201051 release from an HLA\u2013B51\u2013transfected B cell line in the presence of the MICA peptide.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A 9\u2010mer peptide AAAAAIFVI (termed MICA transmembrane [MICA\u2010TM]) was selected as a candidate for the antigenic peptide presented by HLA\u2013B51. A specific T cell response to MICA\u2010TM was detected in 4 patients with BD (29%) but in none of the 15 healthy donors. All 4 responders had HLA\u2013B51 and active disease, and the specific T cell response was lost after the BD\u2010related symptoms disappeared. The MICA\u2010induced T cell response was specifically inhibited by anti\u2013HLA class I antibody or by CD8+ cell depletion. MICA\u2010reactive T cells recognized an HLA\u2013B51\u2013transfected B cell line pulsed with MICA\u2010TM or a B cell line transfected with both HLA\u2013B51 and MICA in the absence of exogenous peptides. Finally, MICA\u2010stimulated T cell lines lysed the HLA\u2013B51\u2013expressing B cell line in the presence of MICA\u2010TM.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>HLA\u2013B51\u2013restricted cytotoxic T lymphocytes autoreactive to MICA may be involved in the pathogenesis of BD.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.20597","type":"journal-article","created":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T21:32:32Z","timestamp":1100295152000},"page":"3658-3662","source":"Crossref","is-referenced-by-count":60,"title":["Autoreactive CD8+ cytotoxic T lymphocytes to major histocompatibility complex class I chain\u2013related gene A in patients with Beh\u00e7et's disease"],"prefix":"10.1002","volume":"50","author":[{"given":"Hidekata","family":"Yasuoka","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuka","family":"Okazaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Kawakami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michito","family":"Hirakata","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hidetoshi","family":"Inoko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yasuo","family":"Ikeda","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Kuwana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,11,4]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199910213411707"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.60.11.996"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380321"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(02)00338-2"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20010956"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.279.5357.1737"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0198-8859(98)00128-1"},{"key":"e_1_2_6_9_2","first-page":"1078","article-title":"Criteria for diagnosis of Beh\u00e7et's disease","volume":"335","author":"International study group for Beh\u00e7et's disease","year":"1990","journal-title":"Lancet"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1997.tb02835.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.4.1298"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9394(14)71396-0"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.052701099"},{"key":"e_1_2_6_14_2","doi-asserted-by":"crossref","first-page":"2257","DOI":"10.4049\/jimmunol.154.5.2257","article-title":"Induction of tumor\u2010reactive CTL from peripheral blood and tumor\u2010infiltrating lymphocytes of melanoma patients by in vitro stimulation with an immunodominant peptide of the human melanoma antigen MART\u20101","volume":"154","author":"Rivoltini L","year":"1995","journal-title":"J Immunol"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.3109\/08830189709116842"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.285.5428.727"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.20597","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.20597","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T05:49:40Z","timestamp":1697521780000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.20597"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11]]},"references-count":15,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2004,11]]}},"alternative-id":["10.1002\/art.20597"],"URL":"https:\/\/doi.org\/10.1002\/art.20597","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11]]}}}