{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T01:26:51Z","timestamp":1791163611099,"version":"4.1.0"},"reference-count":33,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2004,11,4]],"date-time":"2004-11-04T00:00:00Z","timestamp":1099526400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"National Health and Medical Research Council of Australia"},{"DOI":"10.13039\/100008017","name":"Flinders Medical Centre Foundation","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008017","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2004,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The presence, in patients with primary and secondary Sj\u00f6gren's syndrome (SS), of autoantibodies that acutely inhibit M<jats:sub>3<\/jats:sub> muscarinic receptor (M3R)\u2013mediated bladder contractions is difficult to reconcile with the fact that symptoms of detrusor overactivity and other features of cholinergic hyperresponsiveness occur in this disease. This study was undertaken to examine the in vivo effects of these autoantibodies on bladder function by examining bladder responsiveness and compliance following passive transfer of patient IgG to mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Contractile responses of isolated bladder strips both to the muscarinic agonist carbachol and to electrically evoked acetylcholine release were measured 48 hours after injection of mice with patient or control IgG. A whole bladder assay with intact neuronal pathways was developed to assess bladder wall compliance on filling cystometry. Expression of M3R in bladders from IgG\u2010injected mice was assessed by immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Passive transfer of SS IgG with inhibitory anti\u2010M3R activity produced a paradoxical increase in contractile responses of detrusor strips to cholinergic stimulation. Cystometry of whole bladders revealed a corresponding decrease in bladder wall compliance and phasic detrusor contractions upon filling, replicating the urodynamic features of an overactive bladder. The features of cholinergic hyperresponsiveness were associated with increased postsynaptic M3R expression and were reproduced by injecting mice with a rabbit antibody against the second extracellular loop of M3R.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings are consistent with the notion that there is initial inhibition of parasympathetic neurotransmission by antagonistic autoantibodies to M3R, which produces a compensatory increase in M3R expression in vivo. The enhanced cholinergic responses during bladder distention result in detrusor overactivity. We conclude that the overactive bladder associated with SS is an autoantibody\u2010mediated disorder of the autonomic nervous system, which may be part of a wider spectrum of cholinergic hyperresponsiveness.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.20625","type":"journal-article","created":{"date-parts":[[2004,11,12]],"date-time":"2004-11-12T21:26:54Z","timestamp":1100294814000},"page":"3637-3645","source":"Crossref","is-referenced-by-count":69,"title":["Passive transfer of Sj\u00f6gren's syndrome IgG produces the pathophysiology of overactive bladder"],"prefix":"10.1002","volume":"50","author":[{"given":"Fang","family":"Wang","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael W.","family":"Jackson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vicki","family":"Maughan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Cavill","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony J.","family":"Smith","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally A.","family":"Waterman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom P.","family":"Gordon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2004,11,4]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra032662"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9343(03)00257-2"},{"key":"e_1_2_5_4_2","first-page":"2406","article-title":"Increased severity of lower urinary tract symptoms and daytime somnolence in primary Sj\u00f6gren's syndrome","volume":"30","author":"Walker JG","year":"2003","journal-title":"J Rheumatol"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200007)43:7<1647::AID-ANR31>3.0.CO;2-P"},{"key":"e_1_2_5_6_2","first-page":"151","article-title":"Autoantibodies against lacrimal gland M3 muscarinic acetylcholine receptors in patients with primary Sjogren's syndrome","volume":"39","author":"Bacman S","year":"1998","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1996.42748.x"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.11343"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.3109\/08916930109001960"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.62.8.702"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.50.1.36"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1398-9995.2000.00252.x"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keg276"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1038\/labinvest.3780412"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1053\/gast.2002.36057"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780360309"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.0300-9475.2004.01395.x"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-5728(02)00073-5"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1177\/39.6.1709656"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI18180"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1681.1998.tb02169.x"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1523\/JNEUROSCI.21-15-05670.2001"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.11498"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1097\/00019052-200306000-00016"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1007\/PL00004036"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri916"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(03)13583-0"},{"key":"e_1_2_5_28_2","first-page":"125","article-title":"Increased bronchial responsiveness in primary Sjogren's syndrome: a sign of tracheobronchial involvement","volume":"9","author":"La Corte R","year":"1991","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.44.9.610"},{"key":"e_1_2_5_30_2","first-page":"147","article-title":"Manometric assessment of impaired esophageal motor function in primary Sjogren's syndrome","volume":"19","author":"Rosztoczy A","year":"2001","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/0002-9343(82)90539-3"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-5347(05)65403-1"},{"key":"e_1_2_5_33_2","doi-asserted-by":"publisher","DOI":"10.1152\/ajprenal.00187.2002"},{"key":"e_1_2_5_34_2","doi-asserted-by":"publisher","DOI":"10.1080\/00365590310008721"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.20625","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.20625","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T05:51:52Z","timestamp":1697521912000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.20625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,11]]},"references-count":33,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2004,11]]}},"alternative-id":["10.1002\/art.20625"],"URL":"https:\/\/doi.org\/10.1002\/art.20625","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2004,11]]}}}