{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T02:36:08Z","timestamp":1782786968551,"version":"3.54.5"},"reference-count":15,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T00:00:00Z","timestamp":1105056000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>We have previously demonstrated the presence of IgG aggregates modified by oxygen radicals (chlorinated IgG [Cl\u2010IgG]) and peroxynitrite (nitrated IgG [N\u2010IgG]) in synovial fluid of patients with rheumatoid arthritis (RA). A possible explanation for the longstanding chronic inflammatory process in RA is the establishment of an immune response to autoantigens. This study was undertaken to examine whether a T cell response to oxidatively modified IgG contributes to the inflammation in RA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We studied in vitro lymphocyte proliferation and interleukin 2 (IL\u20102) secretion in response to a common antigen (mumps), N\u2010IgG, Cl\u2010IgG, and heat\u2010aggregated IgG (H\u2010IgG) (control) in 15 normal blood donors and 16 RA patients not receiving immunosuppressive drugs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The responses of RA lymphocytes to mumps antigen were significantly lower that those in controls (mean \u00b1 SEM 2,577 \u00b1 217 versus 6,367 \u00b1 365 counts per minute\/well; <jats:italic>P<\/jats:italic> &lt; 0.02). However, whereas in normal donors the cell responses to N\u2010IgG and Cl\u2010IgG were not significantly different than responses to H\u2010IgG (N\u2010IgG\/H\u2010IgG ratio 1.2 \u00b1 0.2, Cl\u2010IgG\/H\u2010IgG 1.5 \u00b1 0.2), the RA lymphocyte responses to N\u2010IgG and Cl\u2010IgG were significantly higher than the responses to H\u2010IgG (N\u2010IgG\/H\u2010IgG 7.4 \u00b1 2.5, Cl\u2010IgG\/H\u2010IgG 4.8 \u00b1 1.2). When ratios in RA cells were compared with normal cell responses as a group, there was a significant difference for both N\u2010IgG (<jats:italic>P<\/jats:italic> &lt; 0.017) and Cl\u2010IgG (<jats:italic>P<\/jats:italic> &lt; 0.014). When selected normal and RA lymphocyte culture supernatants were assayed for IL\u20102 secretion, the increase in IL\u20102 never exceeded 2\u2010fold in normal cell cultures incubated with any of the IgG compared with unstimulated cultures, whereas responses of RA cells, particularly those incubated with N\u2010IgG, were increased (range 2.6\u201315.7\u2010fold) compared with unstimulated controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These results suggest that in RA there are circulating T cells that are responsive to oxidatively modified IgG, a possible pathogenic mechanism contributing to the chronic inflammatory process within the inflamed joint.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.20760","type":"journal-article","created":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T22:40:55Z","timestamp":1105137655000},"page":"80-83","source":"Crossref","is-referenced-by-count":12,"title":["Responses of lymphocytes of patients with rheumatoid arthritis to IgG modified by oxygen radicals or peroxynitrite"],"prefix":"10.1002","volume":"52","author":[{"given":"Susan","family":"Grinnell","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Koji","family":"Yoshida","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hugo E.","family":"Jasin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2005,1,7]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"crossref","first-page":"1918","DOI":"10.4049\/jimmunol.130.4.1918","article-title":"Generation of IgG aggregates by the myeloperoxidase\u2010hydrogen peroxide system","volume":"130","author":"Jasin HE","year":"1983","journal-title":"J Immunol"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI113310"},{"key":"e_1_2_5_4_2","doi-asserted-by":"crossref","first-page":"1422","DOI":"10.4049\/jimmunol.161.3.1422","article-title":"Covalent cross\u2010linking of immune complexes by oxygen radicals and nitrite","volume":"161","author":"Uesugi M","year":"1998","journal-title":"J Immunol"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00917777"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.11.6532"},{"key":"e_1_2_5_7_2","first-page":"1128","volume-title":"Arthritis and allied conditions: a textbook of rheumatology","author":"Jasin HE","year":"2000"},{"key":"e_1_2_5_8_2","first-page":"1086","volume-title":"Arthritis and allied conditions: a textbook of rheumatology","author":"Fox DA","year":"2000"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.2.528"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00916103"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310302"},{"key":"e_1_2_5_12_2","first-page":"645","article-title":"Bacterial lipopolysaccharide\u2010induced immunoglobulin synthesis in blood mononuclear cells partially depleted of monocytes","volume":"47","author":"Sager DS","year":"1982","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780190301"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200001)43:1<155::AID-ANR20>3.0.CO;2-3"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.56.5.317"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/0014-5793(94)00722-5"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.20760","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.20760","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,11,23]],"date-time":"2023-11-23T03:21:17Z","timestamp":1700709677000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.20760"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,1]]},"references-count":15,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2005,1]]}},"alternative-id":["10.1002\/art.20760"],"URL":"https:\/\/doi.org\/10.1002\/art.20760","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,1]]}}}