{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,24]],"date-time":"2026-03-24T06:14:51Z","timestamp":1774332891850,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T00:00:00Z","timestamp":1105056000000},"content-version":"vor","delay-in-days":6,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2005,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To examine the glycosaminoglycan (GAG) content in cartilage and that in synovial fluid and determine whether they are associated, in patients with an acute anterior cruciate ligament (ACL) injury.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Twenty\u2010four patients (14 of whom were male) with a mean age of 27 years (range 17\u201340 years) were assessed with delayed gadolinium\u2010enhanced magnetic resonance imaging (MRI) of cartilage an average of 3 weeks after an ACL rupture and compared with 24 healthy volunteers. Two hours after an intravenous injection of Gd\u2010DTPA<jats:sup>2\u2212<\/jats:sup> (0.3 mmoles\/kg body weight), quantitative measurements of the T1 relaxation time (T1<jats:sub>Gd<\/jats:sub> [T1 relaxation time in the presence of Gd\u2010DTPA]) were made in lateral and medial femoral weight\u2010bearing cartilage. In the patients, synovial fluid was aspirated immediately before the MRI, and GAG was analyzed using dye precipitation with Alcian blue.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Fifteen of the 24 patients had an isolated bone bruise in the lateral femoral condyle, where the cartilage T1<jats:sub>Gd<\/jats:sub> was shorter than that in the controls (mean \u00b1 SD 385 \u00b1 83 msec and 445 \u00b1 41 msec, respectively; <jats:italic>P<\/jats:italic> = 0.004), consistent with decreased GAG content. However, the T1<jats:sub>Gd<\/jats:sub> was also decreased in the medial femoral cartilage, where bone bruises were rare (376 \u00b1 76 msec in patients versus 428 \u00b1 38 msec in controls; <jats:italic>P<\/jats:italic> = 0.006). The mean \u00b1 SD synovial fluid GAG concentration in patients was 157 \u00b1 86 \u03bcg\/ml and showed a positive correlation with the T1<jats:sub>Gd<\/jats:sub> (r = 0.49, <jats:italic>P<\/jats:italic> = 0.02).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study indicates that an ACL injury causes posttraumatic edema of the lateral femoral cartilage but initializes a generalized biochemical change within the knee that leads to GAG loss from both lateral and medial femoral cartilage. In cartilage with a high GAG content (long T1<jats:sub>Gd<\/jats:sub>), more GAG is released into the synovial fluid, suggesting that cartilage quality is a factor to consider when interpreting cartilage biomarkers of metabolism.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.20795","type":"journal-article","created":{"date-parts":[[2005,1,7]],"date-time":"2005-01-07T21:48:59Z","timestamp":1105134539000},"page":"120-127","source":"Crossref","is-referenced-by-count":121,"title":["Cartilage glycosaminoglycan loss in the acute phase after an anterior cruciate ligament injury: Delayed gadolinium\u2010enhanced magnetic resonance imaging of cartilage and synovial fluid analysis"],"prefix":"10.1002","volume":"52","author":[{"given":"Carl Johan","family":"Tiderius","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lars 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