{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T12:55:20Z","timestamp":1790686520687,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T00:00:00Z","timestamp":1109894400000},"content-version":"vor","delay-in-days":3,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2005,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Several hypotheses have been proposed to explain the strong association between HLA\u2013B27 and ankylosing spondylitis (AS). Among these, the arthritogenic peptide theory proposes that certain B27 subtype alleles bind specific arthritogenic peptide(s) due to their unique amino acid anchor residues. Cartilage antigens have been discussed as candidate targets for the immune response in AS. The recognition of HLA\u2013B27\u2013peptide complexes by self\u2010reactive CD8+ T cells might contribute to joint\u2010specific tissue damage. Therefore, we investigated the presence of autoreactive CD8+ T cells specific for cartilage\u2010derived peptides in patients with AS.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>An HLA\u2013B27\u2013binding prediction program and a proteasome\u2010cutting prediction program for the human 20S proteasome were used to screen 18 human cartilage proteins for potentially immunogenic nonamer peptides. The peptides identified were used to stimulate peripheral blood mononuclear cells from 20 HLA\u2013B27\u2013positive patients with AS and synovial fluid (SF) mononuclear cells from 7 HLA\u2013B27\u2013positive patients with AS. Activation of T cells was measured by antigen\u2010specific intracellular cytokine staining and quantified by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>From the screening analysis, we identified 121 nonamer peptides. Of these, 1 peptide derived from type II collagen and 1 from type VI collagen were stimulatory for peripheral blood CD8+ T cells in only 1 of 20 patients. However, in 4 of 7 SF samples the same type VI collagen\u2013derived nonamer peptide stimulated SF CD8+ T cells, but none of the other peptides was stimulatory. This CD8+ T cell response could be blocked by an anti\u2013HLA\u2013B27 antibody, confirming an HLA\u2013B27\u2013restricted immune response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings suggest that cartilage\u2010directed cellular autoimmunity might play an important role in joint\u2010specific tissue damage in patients with AS. Future research is necessary to determine whether the identified peptide is of pathogenetic relevance.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.20948","type":"journal-article","created":{"date-parts":[[2005,3,4]],"date-time":"2005-03-04T15:54:15Z","timestamp":1109951655000},"page":"892-901","source":"Crossref","is-referenced-by-count":105,"title":["HLA\u2013B27\u2013restricted CD8+ T cell response to cartilage\u2010derived self peptides in ankylosing 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