{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T15:06:26Z","timestamp":1772895986979,"version":"3.50.1"},"reference-count":49,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,6,2]],"date-time":"2005-06-02T00:00:00Z","timestamp":1117670400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Mutations in fibrillin 1, a key component of extracellular microfibrils, are associated with connective tissue disorders such as Marfan's syndrome or skin fibrosis in the tight skin mouse model of scleroderma. Previous studies have suggested that fibrillin 1 mediates skin fibrosis via its interface with associated microfibrillar proteins and type I collagen; in particular, microfibril\u2010associated glycoprotein 2 (MAGP\u20102), an extracellular matrix protein that binds to fibrillins and the \u03b1v\u03b23 integrin, is increased in TSK mouse and human scleroderma skin. Because the function of MAGP\u20102 in the biologic processes of the matrix remains unknown, this study investigated whether MAGP\u20102 regulates type I collagen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Fibroblast cultures conditionally overexpressing MAGP\u20102 were developed. Cells were analyzed by Western blotting, Northern blotting, pulse\u2010chase analysis, and immunofluorescence to assess the effect of MAGP\u20102 on type I collagen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cells overexpressing MAGP\u20102 formed increased MAGP\u20102 matrix and showed a 3\u2010fold increase in intracellular type I procollagen. This increase was associated with increased levels of type I collagen in the medium and matrix. Increased type I collagen colocalized with the MAGP\u20102 matrix. MAGP\u20102 overexpression had no effect on type I procollagen messenger RNA, but markedly increased the half\u2010life of type I procollagen. MAGP\u20102 induced type I collagen even under conditions in which no MAGP\u20102 matrix was detectable, and did not require the presence of the RGD motif of MAGP\u20102 in its integrin\u2010binding site.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study shows that MAGP\u20102 stabilizes type I procollagen, identifying an important function of MAGP\u20102 in extracellular matrix homeostasis. It also suggests that MAGP\u20102 might mediate skin fibrosis in TSK mice and in patients with scleroderma.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.21059","type":"journal-article","created":{"date-parts":[[2005,6,3]],"date-time":"2005-06-03T13:03:21Z","timestamp":1117803801000},"page":"1812-1823","source":"Crossref","is-referenced-by-count":29,"title":["Increased expression of type I collagen induced by microfibril\u2010associated glycoprotein 2: Novel mechanistic insights into the molecular basis of dermal fibrosis in scleroderma"],"prefix":"10.1002","volume":"52","author":[{"given":"Raphael","family":"Lemaire","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph H.","family":"Korn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J. Michael","family":"Shipley","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Lafyatis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,2]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4684-9093-0_2"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-775170-2.50014-7"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-363710-9.50009-5"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-2828(90)90087-I"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/1357-2725(95)00028-N"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.103.6.2499"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.124.5.855"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/352330a0"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.1380010504"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4757-9062-7_4"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng1097-218"},{"key":"e_1_2_5_13_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.7.3819"},{"key":"e_1_2_5_14_2","first-page":"791","article-title":"Extracellular microfibrils are increased in localized and systemic scleroderma skin","volume":"64","author":"Fleischmajer R","year":"1991","journal-title":"Lab Invest"},{"key":"e_1_2_5_15_2","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1001\/archderm.1972.01620040031005","article-title":"Alteration of subcutaneous tissue in systemic scleroderma","volume":"105","author":"Fleischmajer R","year":"1972","journal-title":"Arch Dermatol"},{"key":"e_1_2_5_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199810)41:10<1729::AID-ART5>3.0.CO;2-8"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200104)44:4<893::AID-ANR146>3.0.CO;2-3"},{"key":"e_1_2_5_18_2","first-page":"493","article-title":"Tight\u2010skin, a new mutation of the mouse causing excessive growth of connective tissue and skeleton","volume":"82","author":"Green MC","year":"1976","journal-title":"Am J Pathol"},{"key":"e_1_2_5_19_2","doi-asserted-by":"publisher","DOI":"10.1111\/1523-1747.ep12535041"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1101\/gr.6.4.300"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M206363200"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M104533200"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M313672200"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1091\/mbc.11.5.1499"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M109540200"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.36.22817"},{"key":"e_1_2_5_27_2","doi-asserted-by":"publisher","DOI":"10.1177\/002215549804600802"},{"key":"e_1_2_5_28_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.271.2.1096"},{"key":"e_1_2_5_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.19.13060"},{"key":"e_1_2_5_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(00)00100-1"},{"key":"e_1_2_5_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20053"},{"key":"e_1_2_5_32_2","doi-asserted-by":"publisher","DOI":"10.1007\/s003350010036"},{"key":"e_1_2_5_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10621"},{"key":"e_1_2_5_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.272.30.19059"},{"key":"e_1_2_5_35_2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.65.1.75"},{"key":"e_1_2_5_36_2","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4615-3770-0_8"},{"key":"e_1_2_5_37_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.69.4.892"},{"key":"e_1_2_5_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/352337a0"},{"key":"e_1_2_5_39_2","doi-asserted-by":"publisher","DOI":"10.1038\/352334a0"},{"key":"e_1_2_5_40_2","doi-asserted-by":"publisher","DOI":"10.1006\/jmbi.1996.0237"},{"key":"e_1_2_5_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(95)90294-5"},{"key":"e_1_2_5_42_2","doi-asserted-by":"publisher","DOI":"10.1006\/bbrc.2000.2173"},{"key":"e_1_2_5_43_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M100933200"},{"key":"e_1_2_5_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/B978-0-12-487425-1.50013-9"},{"key":"e_1_2_5_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/0006-291X(73)90961-3"},{"key":"e_1_2_5_46_2","doi-asserted-by":"publisher","DOI":"10.1038\/33573"},{"key":"e_1_2_5_47_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0945-053X(98)90119-6"},{"key":"e_1_2_5_48_2","doi-asserted-by":"publisher","DOI":"10.1021\/ja034015j"},{"key":"e_1_2_5_49_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.27.18843"},{"key":"e_1_2_5_50_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0344-0338(11)80995-7"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.21059","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.21059","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:35:06Z","timestamp":1697060106000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.21059"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,6]]},"references-count":49,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2005,6]]}},"alternative-id":["10.1002\/art.21059"],"URL":"https:\/\/doi.org\/10.1002\/art.21059","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,6]]}}}