{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T10:56:47Z","timestamp":1760266607153},"reference-count":25,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2005,6,2]],"date-time":"2005-06-02T00:00:00Z","timestamp":1117670400000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2005,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Beh\u00e7et's disease is generally considered to be a multifactorial disease with important genetic and environmental components. A strong association between an HLA class I antigen, HLA\u2013B51, and Beh\u00e7et's disease has long been known. However, analysis of multicase families has suggested a substantial contribution of non\u2010HLA loci. The aim of this study was to perform a whole\u2010genome linkage analysis for identification of other susceptibility loci for Beh\u00e7et's disease in multicase families.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The study group comprised a total of 193 individuals (90 male, 103 female) from 28 multicase families of Turkish origin; 83 of the subjects (50 males, 33 females) fulfilled the International Study Group criteria for Beh\u00e7et's disease. Three hundred ninety\u2010five highly informative microsatellite markers spanning the genome were genotyped using fluorescent polymerase chain reaction primers and a fully automated electrophoresis platform. After the first analysis, 33 additional markers that were located close to the peak linkage areas were genotyped in all individuals. Nonparametric multipoint linkage analysis was carried out using GeneHunter version 2.1 software.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Evidence for linkage (<jats:italic>P<\/jats:italic> \u2264 0.05) was obtained in 16 chromosome regions: 1p36, 4p15, 5q12, 5q23, 6p22\u201324, 6q16, 6q25\u201326, 7p21, 10q24, 12p12\u201313, 12q13, 16q12, 16q21\u201323, 17p13, 20q12\u201313, and Xq26\u201328. After the addition of further markers, the maximum nonparametric linkage score increased from 3.5 to 3.94 at 12p12\u201313 (D12S77; <jats:italic>P<\/jats:italic> = 0.0002) and from 3.07 to 3.70 at 6p22\u201324 (D6S285; <jats:italic>P<\/jats:italic> = 0.0005).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study is the first systematic genome screen in Beh\u00e7et's disease and provides evidence of linkage to several non\u2010HLA susceptibility loci in a cohort of Turkish multicase families. This represents the first step toward the identification of novel Beh\u00e7et's disease susceptibility genes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.21060","type":"journal-article","created":{"date-parts":[[2005,6,3]],"date-time":"2005-06-03T13:04:34Z","timestamp":1117803874000},"page":"1836-1842","source":"Crossref","is-referenced-by-count":89,"title":["Whole\u2010genome screening for susceptibility genes in multicase families with Beh\u00e7et's disease"],"prefix":"10.1002","volume":"52","author":[{"given":"Jumana","family":"Karasneh","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmet","family":"G\u00fcl","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"William E.","family":"Ollier","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J.","family":"Silman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jane","family":"Worthington","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,2]]},"reference":[{"key":"e_1_2_5_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199910213411707"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1399-0039.1999.540301.x"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-4362.2003.01893.x"},{"issue":"24","key":"e_1_2_5_5_2","first-page":"S6","article-title":"Beh\u00e7et's disease: an update on the pathogenesis","volume":"19","author":"Gul A","year":"2001","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-3476(99)70333-1"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.59.8.622"},{"key":"e_1_2_5_8_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.45.4.265"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/ajmg.a.20136"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.57.1.45"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1001\/archopht.1982.01030040433013"},{"key":"e_1_2_5_12_2","doi-asserted-by":"publisher","DOI":"10.1086\/302364"},{"key":"e_1_2_5_13_2","first-page":"3702","article-title":"Localization of the pathogenic gene of Beh\u00e7et's disease by microsatellite analysis of three different populations","volume":"41","author":"Mizuki N","year":"2000","journal-title":"Invest Ophthalmol Vis Sci"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200101)44:1<239::AID-ANR31>3.0.CO;2-X"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200111)44:11<2693::AID-ART449>3.0.CO;2-M"},{"key":"e_1_2_5_16_2","first-page":"1078","article-title":"Criteria for diagnosis of Beh\u00e7et's disease","volume":"335","author":"International Study Group for Beh\u00e7et's Disease","year":"1990","journal-title":"Lancet"},{"key":"e_1_2_5_17_2","first-page":"543","article-title":"Estimating the power of a proposed linkage study for a complex genetic trait","volume":"44","author":"Ploughman LM","year":"1989","journal-title":"Am J Hum Genet"},{"key":"e_1_2_5_18_2","doi-asserted-by":"publisher","DOI":"10.1086\/301904"},{"key":"e_1_2_5_19_2","first-page":"1347","article-title":"Parametric and nonparametric linkage analysis: a unified multipoint approach","volume":"58","author":"Kruglyak L","year":"1996","journal-title":"Am J Hum Genet"},{"key":"e_1_2_5_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh561"},{"key":"e_1_2_5_21_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10147"},{"key":"e_1_2_5_22_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199804)41:4<588::AID-ART5>3.0.CO;2-0"},{"key":"e_1_2_5_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/302677"},{"key":"e_1_2_5_24_2","doi-asserted-by":"publisher","DOI":"10.1007\/s002510050468"},{"key":"e_1_2_5_25_2","doi-asserted-by":"publisher","DOI":"10.1086\/301886"},{"key":"e_1_2_5_26_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80721-7"}],"container-title":["Arthritis &amp; 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