{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,14]],"date-time":"2026-04-14T23:10:57Z","timestamp":1776208257014,"version":"3.50.1"},"reference-count":16,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,6,28]],"date-time":"2005-06-28T00:00:00Z","timestamp":1119916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Rheumatoid arthritis (RA) is characterized by inflammation and destruction of synovial joints. Fibroblast\u2010like synoviocytes (FLS) harvested from synovial tissue of patients with RA can invade normal human cartilage in severe combined immunodeficient (SCID) mice and Matrigel basement membrane matrix in vitro. This study was undertaken to investigate the association of these in vitro characteristics with disease characteristics in patients with RA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Synovial tissue samples from 72 RA and 49 osteoarthritis (OA) patients were obtained. Samples of different joints were collected from 7 patients with RA. The FLS invasiveness in Matrigel was studied, and the intraindividual and interindividual differences were compared. From the patients with FLS who exhibited the most extreme differences in in vitro ingrowth (most and least invasive FLS), radiographs of the hands and feet were collected and scored according to the Sharp\/van der Heijde method to determine the relationship between in vitro invasion data and estimated yearly joint damage progression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>FLS from patients with RA were more invasive than FLS from patients with OA (<jats:italic>P<\/jats:italic> &lt; 0.001). The mean intraindividual variation in FLS invasion was much less than the mean interindividual variation (mean \u00b1 SD 1,067 \u00b1 926 and 3,845 \u00b1 2,367 for intraindividual and interindividual variation, respectively; <jats:italic>P<\/jats:italic> = 0.035), which shows that the level of FLS invasion is a patient characteristic. The mean \u00b1 SEM Sharp score on radiographs of the hands or feet divided by the disease duration was 4.4 \u00b1 1.1 units per year of disease duration in patients with the least invasive FLS (n = 9), which was much lower compared with the 21.8 \u00b1 3.1 units per year of disease duration in patients with the most invasive FLS (n = 9) (<jats:italic>P<\/jats:italic> &lt; 0.001).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The ex vivo invasive behavior of FLS from RA patients is associated with the rate of joint destruction and is a patient characteristic, given the much smaller intraindividual than interindividual FLS variation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.21118","type":"journal-article","created":{"date-parts":[[2005,6,28]],"date-time":"2005-06-28T14:48:28Z","timestamp":1119970108000},"page":"1999-2002","source":"Crossref","is-referenced-by-count":127,"title":["Invasiveness of fibroblast\u2010like synoviocytes is an individual patient characteristic associated with the rate of joint destruction in patients with rheumatoid arthritis"],"prefix":"10.1002","volume":"52","author":[{"given":"Tanja C. A.","family":"Tolboom","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annette H. M.","family":"van der Helm\u2010Van Mil","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rob G. H. H.","family":"Nelissen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ferdinand C.","family":"Breedveld","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ren\u00e9 E. M.","family":"Toes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom W. J.","family":"Huizinga","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2005,6,28]]},"reference":[{"key":"e_1_2_5_2_2","first-page":"1607","article-title":"Synovial fibroblasts of patients with rheumatoid arthritis attach to and invade normal human cartilage when engrafted into SCID mice","volume":"149","author":"Muller\u2010Ladner U","year":"1996","journal-title":"Am J Pathol"},{"key":"e_1_2_5_3_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114011"},{"key":"e_1_2_5_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.11.6610"},{"key":"e_1_2_5_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.11.975"},{"key":"e_1_2_5_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0934-8832(11)80142-3"},{"key":"e_1_2_5_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199905)42:5<948::AID-ANR13>3.0.CO;2-L"},{"key":"e_1_2_5_8_2","first-page":"2143","article-title":"Apoptosis in rheumatoid arthritis: p53 overexpression in rheumatoid arthritis synovium","volume":"149","author":"Firestein GS","year":"1996","journal-title":"Am J Pathol"},{"key":"e_1_2_5_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310302"},{"key":"e_1_2_5_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(89)92442-2"},{"key":"e_1_2_5_11_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.suppl_2.ii64"},{"key":"e_1_2_5_12_2","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1016\/S0889-857X(21)00462-2","article-title":"Oncogenes in rheumatoid arthritis","volume":"21","author":"Muller\u2010Ladner U","year":"1995","journal-title":"Rheum Dis Clin North Am"},{"issue":"37","key":"e_1_2_5_13_2","first-page":"S314","article-title":"Expression and localization of oncoproteins in rheumatoid synovial tissues","author":"Nakagawa Y","year":"1994","journal-title":"Arthritis Rheum"},{"key":"e_1_2_5_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780370210"},{"key":"e_1_2_5_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20461"},{"key":"e_1_2_5_16_2","first-page":"1091","article-title":"Regulation of synoviocyte proliferation, apoptosis, and invasion by the p53 tumor suppressor gene","volume":"152","author":"Aupperle KR","year":"1998","journal-title":"Am J Pathol"},{"key":"e_1_2_5_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200103)44:3<676::AID-ANR117>3.0.CO;2-6"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.21118","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.21118","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,17]],"date-time":"2023-10-17T19:50:48Z","timestamp":1697572248000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.21118"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2005,6,28]]},"references-count":16,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2005,7]]}},"alternative-id":["10.1002\/art.21118"],"URL":"https:\/\/doi.org\/10.1002\/art.21118","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2005,6,28]]}}}