{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T02:23:38Z","timestamp":1782786218426,"version":"3.54.5"},"reference-count":56,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2005,6,28]],"date-time":"2005-06-28T00:00:00Z","timestamp":1119916800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2005,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine whether prolongation of the inflammatory reaction in patients with Beh\u00e7et's disease (BD) is related to apoptosis resistance and is associated with the up\u2010regulation of antiapoptotic factors.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The percentage of cell death was evaluated by flow cytometry in peripheral blood mononuclear cells from 35 patients with BD and 30 healthy volunteers. The expression levels of antiapoptotic factors and NF\u2010\u03baB regulatory proteins were measured using Western blotting and immunohistochemical analyses. To down\u2010regulate NF\u2010\u03baB nuclear translocation, BD T lymphocytes were exposed in vitro to thalidomide and subjected to transfection with NF\u2010\u03baB small interfering RNA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Although CD95 is highly expressed in BD T cells, the absence of sensitivity to CD95\u2010induced apoptosis observed may be attributable to the inhibitory action of antiapoptotic genes. Immunoblot analysis for major antiapoptotic proteins showed considerable up\u2010regulation of the short form of cellular FLIP (cFLIP) and Bcl\u2010x<jats:sub>L<\/jats:sub> in BD activated T cells, while levels of Bcl\u20102, caspase 3, and caspase 8 in activated T cells from patients with BD were comparable with those in activated T cells from normal donors. Moreover, expression of IKK and I\u03baB was up\u2010regulated, whereas NF\u2010\u03baB translocated to the nucleus in BD T cells, suggesting that NF\u2010\u03baB activation may modulate the expression of antiapoptotic genes. Interestingly, thalidomide and NF\u2010\u03baB small interfering RNA down\u2010regulated cFLIP and Bcl\u2010x<jats:sub>L<\/jats:sub> expression levels and sensitized BD activated T cells to CD95\u2010induced apoptosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Taken together, these results indicate that NF\u2010\u03baB contributes to the regulation of the apoptosis\u2010related factors and death receptors leading to apoptosis resistance in BD T cell subsets. Our results suggest that NF\u2010\u03baB plays a crucial role in the pathogenesis of BD, and that its pharmacologic control could represent a key strategy in modulating specific immune\u2010mediated disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.21145","type":"journal-article","created":{"date-parts":[[2005,6,28]],"date-time":"2005-06-28T15:50:04Z","timestamp":1119973804000},"page":"2179-2191","source":"Crossref","is-referenced-by-count":55,"title":["NF\u2010\u03baB protects Beh\u00e7et's disease T cells against CD95\u2010induced apoptosis up\u2010regulating antiapoptotic 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