{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:27:14Z","timestamp":1759937234715},"reference-count":48,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2005,12,29]],"date-time":"2005-12-29T00:00:00Z","timestamp":1135814400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2006,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Both the genesis and outgrowth of extranodal marginal\u2010zone B cell lymphomas (MZLs) of the mucosa\u2010associated lymphoid tissue (MALT) type are generally thought to represent antigen\u2010driven processes. We undertook this study to analyze lymphoma progression and dissemination outside of the MALT\u2010type lesions.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Histopathologic and Ig heavy\u2010 and light\u2010chain variable\u2010region gene (V<jats:sub>H\/L<\/jats:sub>) analyses were performed in sequential tissue samples from a patient with primary Sj\u00f6gren's syndrome (SS) with glandular (parotid) manifestations and subsequent nodal dissemination of a low\u2010grade MZL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>This MZL expressed a CD20+,CD27+,sIgM\/\u03ba+,IgD\u2212,CD5\u2212,CD10\u2212,Bcl\u20106\u2212,CD23\u2212,p53\u2212,p21\u2212,MDM2\u2212 phenotype and mutated V<jats:sub>H<\/jats:sub>1\u201369\/D2\u201321\/J<jats:sub>H<\/jats:sub>4\u03b1\u2013V<jats:sub>\u03ba<\/jats:sub>A27\/J<jats:sub>\u03ba<\/jats:sub>2 Ig rearrangements. Notably, circulating lymphoma cells from the parotid glands occurred transiently in the patient's blood, as detected by single\u2010cell polymerase chain reaction. In addition, 2 minor B cell clones (clones 2 and 3, with V<jats:sub>H<\/jats:sub>3\u201307\/D3\u201322\/J<jats:sub>H<\/jats:sub>3b\u2013V<jats:sub>\u03bb<\/jats:sub>3L\/J<jats:sub>\u03bb<\/jats:sub>2\/3 and V<jats:sub>H<\/jats:sub>3\u201364\/D3\u201303\/J<jats:sub>H<\/jats:sub>2\u2013V<jats:sub>\u03ba<\/jats:sub>A19\/J<jats:sub>\u03ba<\/jats:sub>2 rearrangements, respectively) were also detected in the parotid glands and blood, and 1 of these (clone 2) was also detected in the lymph nodes. Ig V<jats:sub>H\/L<\/jats:sub> analyses revealed ongoing (antigen\u2010driven) mutations of the glandular lymphoma rearrangements, but an invariant mutation pattern of their nodal counterparts.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data indicate coexpansion and transient (re)circulation of the lymphoma clone and 2 additional glandular B cell clones in a primary SS\u2013associated extranodal MZL. Combined histologic and molecular features of the nodal lymphoma subclone reflect a process of \u201cfollicular colonization\u201d that eventually froze the mutation machinery after accumulation of additional (antigen\u2010driven) Ig V<jats:sub>H\/L<\/jats:sub> mutations.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.21558","type":"journal-article","created":{"date-parts":[[2005,12,29]],"date-time":"2005-12-29T18:22:53Z","timestamp":1135880573000},"page":"127-137","source":"Crossref","is-referenced-by-count":18,"title":["Dissemination of a Sj\u00f6gren's syndrome\u2013associated extranodal marginal\u2010zone B cell lymphoma: Circulating lymphoma cells and invariant mutation pattern of nodal Ig heavy\u2010 and light\u2010chain variable\u2010region gene rearrangements"],"prefix":"10.1002","volume":"54","author":[{"given":"A.","family":"Hansen","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"K.","family":"Reiter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A.","family":"Pruss","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C.","family":"Loddenkemper","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.","family":"Kaufmann","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"A. 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