{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,11]],"date-time":"2026-03-11T13:22:16Z","timestamp":1773235336348,"version":"3.50.1"},"reference-count":37,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2006,9,28]],"date-time":"2006-09-28T00:00:00Z","timestamp":1159401600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2006,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Arthritis is one of the hallmarks of late\u2010stage Lyme disease. Previous studies have shown that infection with <jats:italic>Borrelia burgdorferi<\/jats:italic>, the causative agent of Lyme disease, results in degradation of proteoglycans and collagen in cartilage. <jats:italic>B burgdorferi<\/jats:italic> do not appear to produce any exported proteases capable of digesting proteoglycans and collagen, but instead, induce and activate host proteases, such as matrix metalloproteinases (MMPs), which results in cartilage degradation. The role of aggrecanases in Lyme arthritis has not yet been determined. We therefore sought to delineate the contribution of aggrecanases to joint destruction in Lyme arthritis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We examined the expression patterns of aggrecanases 1 and 2 (ADAMTS 4 and 5, respectively) in <jats:italic>B burgdorferi<\/jats:italic>\u2013infected primary human chondrocyte cell cultures, in synovial fluid samples from patients with active Lyme arthritis, and in the joints of mice by real\u2010time quantitative reverse transcription\u2013polymerase chain reaction and immunoblotting techniques. Bovine cartilage explants were used to determine the role of aggrecanases in <jats:italic>B burgdorferi<\/jats:italic>\u2013induced cartilage degradation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>ADAMTS\u20104, but not ADAMTS\u20105, was induced in human chondrocytes infected with <jats:italic>B burgdorferi<\/jats:italic>. The active forms of ADAMTS\u20104 were increased in synovial fluid samples from patients with active Lyme arthritis and were elevated in the joints of mice infected with <jats:italic>B burgdorferi<\/jats:italic>. Using cartilage explant models of Lyme arthritis, it appeared that the cleavage of aggrecan was predominantly mediated by \u201caggrecanases\u201d rather than MMPs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The induction of ADAMTS\u20104 by <jats:italic>B burgdorferi<\/jats:italic> results in the cleavage of aggrecan, which may be an important first step that leads to permanent degradation of cartilage.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.22128","type":"journal-article","created":{"date-parts":[[2006,9,28]],"date-time":"2006-09-28T20:06:07Z","timestamp":1159473967000},"page":"3319-3329","source":"Crossref","is-referenced-by-count":29,"title":["Role of aggrecanase 1 in Lyme arthritis"],"prefix":"10.1002","volume":"54","author":[{"given":"Aruna K.","family":"Behera","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ethan","family":"Hildebrand","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jon","family":"Szafranski","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Han\u2010Hwa","family":"Hung","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alan J.","family":"Grodzinsky","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Lafyatis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alisa E.","family":"Koch","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert","family":"Kalish","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"George","family":"Perides","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Allen C.","family":"Steere","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Linden T.","family":"Hu","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2006,9,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.7326\/0003-4819-107-5-725"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780310405"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200106)44:6<1401::AID-ART234>3.0.CO;2-S"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1086\/322000"},{"key":"e_1_2_6_6_2","first-page":"5","volume-title":"Articular cartilage and osteoarthritis","author":"Hardingham T","year":"1991"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)31499-6"},{"key":"e_1_2_6_8_2","doi-asserted-by":"crossref","first-page":"8198","DOI":"10.1016\/S0021-9258(18)92961-3","article-title":"Analysis of the catabolism of aggrecan in cartilage explants by quantitation of peptides from the three globular domains","volume":"266","author":"Sandy JD","year":"1991","journal-title":"J Biol Chem"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(92)90144-L"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1042\/bj2840589"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.270.6.2550"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1042\/0264-6021:3440061"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.273.28.17451"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.09.004"},{"key":"e_1_2_6_15_2","doi-asserted-by":"crossref","first-page":"1008","DOI":"10.1016\/S0021-9258(18)48387-1","article-title":"Identification of a stromelysin cleavage site within the interglobular domain of human aggrecan: evidence for proteolysis at this site in vivo in human articular cartilage","volume":"267","author":"Flannery CR","year":"1992","journal-title":"J Biol Chem"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780360906"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI115742"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1053\/joca.2001.0427"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.73.1.126-134.2005"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.72.5.2864-2871.2004"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.74.3.1462-1470.2006"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1086\/315357"},{"issue":"13","key":"e_1_2_6_24_2","first-page":"1","article-title":"Case definitions for public health surveillance","volume":"39","author":"Wharton M","year":"1990","journal-title":"MMWR Recomm Rep"},{"key":"e_1_2_6_25_2","first-page":"590","article-title":"Recommendations for test performance and interpretation from the Second National Conference on Serologic Diagnosis of Lyme Disease","volume":"44","year":"1995","journal-title":"MMWR Morb Mortal Wkly Rep"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM199401273300401"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1006\/abbi.1997.0205"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/0304-4165(86)90306-5"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M312797200"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.10.023"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2004.09.003"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1097\/01.cad.0000176504.86551.5c"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1023\/A:1011183905848"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199906)42:6<1128::AID-ANR9>3.0.CO;2-2"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.12.6352"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1267"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M303737200"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.9.5777"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.22128","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.22128","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T21:31:04Z","timestamp":1698787864000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.22128"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9,28]]},"references-count":37,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2006,10]]}},"alternative-id":["10.1002\/art.22128"],"URL":"https:\/\/doi.org\/10.1002\/art.22128","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9,28]]}}}