{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T07:47:09Z","timestamp":1791100029040,"version":"4.1.0"},"reference-count":19,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2006,12,28]],"date-time":"2006-12-28T00:00:00Z","timestamp":1167264000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2007,1]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Familial Mediterranean fever (FMF) is associated with more than 70 missense mutations in the <jats:italic>MEFV<\/jats:italic> gene. The purpose of this study was to investigate the relative expression of messenger RNA (mRNA) for the <jats:italic>MEFV<\/jats:italic> gene in peripheral blood leukocytes (PBLs) obtained from patients with FMF during attacks of acute abdominal inflammation as well as during asymptomatic periods.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We studied 16 patients with FMF during an attack of acute peritonitis and 17 otherwise healthy individuals who were undergoing surgery because of acute appendicitis. Blood samples were collected from both groups of patients during both acute inflammatory and asymptomatic periods. Relative levels of <jats:italic>MEFV<\/jats:italic> mRNA in PBLs were detected with real\u2010time reverse transcriptase\u2013polymerase chain reaction using LightCycler, with 2 sets of primers for the <jats:italic>MEFV<\/jats:italic> gene (exons 7\u201310 and exons 2\u20133) and with primers for <jats:italic>CIAS1<\/jats:italic> and <jats:italic>PSTPIP1<\/jats:italic> genes. Expression levels were compared with \u03b2<jats:sub>2<\/jats:sub>\u2010microglobulin as an internal control.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>MEFV<\/jats:italic> expression was reduced in FMF patients during asymptomatic periods as compared with the non\u2010FMF controls (<jats:italic>P<\/jats:italic> &lt; 0.001). We observed a further decrease in <jats:italic>MEFV<\/jats:italic> expression in FMF patients during periods of inflammation (<jats:italic>P<\/jats:italic> = 0.01). Reduced levels of <jats:italic>MEFV<\/jats:italic> mRNA were also noted during the preoperative period as compared with asymptomatic periods in control patients with acute appendicitis (<jats:italic>P<\/jats:italic> = 0.01). <jats:italic>CIAS1<\/jats:italic> expression in PBLs from patients with FMF was also found to be lower than that in the control patients. However, <jats:italic>CIAS1<\/jats:italic> expression did not change with acute inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study confirmed that reduced expression of the <jats:italic>MEFV<\/jats:italic> gene is associated with inflammation and that it may be one of the pathogenic mechanisms of the attacks of inflammation in FMF patients, along with disease\u2010associated variations in pyrin.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.22320","type":"journal-article","created":{"date-parts":[[2006,12,29]],"date-time":"2006-12-29T01:18:46Z","timestamp":1167355126000},"page":"345-350","source":"Crossref","is-referenced-by-count":23,"title":["Association between reduced levels of <i>MEFV<\/i> messenger RNA in peripheral blood leukocytes and acute inflammation"],"prefix":"10.1002","volume":"56","author":[{"given":"Duran","family":"\u00dcstek","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cumhur G\u00f6khan","family":"Ekmekci","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fatih","family":"Sel\u00e7ukbiricik","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aris","family":"Cakiris","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basar","family":"Oku","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Burcak","family":"Vural","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hakan","family":"Yanar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Korhan","family":"Taviloglu","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ugur","family":"\u00d6zbek","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmet","family":"G\u00fcl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,1,2]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/bor.0000174210.78449.6b"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)80539-5"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/ng0997-25"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1788"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.20080"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.ejhg.5200658"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.febslet.2005.11.076"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.emboj.7600994"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1038\/nsmb1034"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0602081103"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1097-2765(03)00056-X"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10575"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780401023"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1086\/302459"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0006-291X(03)00221-3"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/9.20.3001"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20600"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1086\/341357"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kei140"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.22320","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.22320","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T21:21:57Z","timestamp":1697059317000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.22320"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,1]]},"references-count":19,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2007,1]]}},"alternative-id":["10.1002\/art.22320"],"URL":"https:\/\/doi.org\/10.1002\/art.22320","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,1]]}}}