{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T09:43:12Z","timestamp":1790934192682,"version":"4.1.0"},"reference-count":46,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2007,3,28]],"date-time":"2007-03-28T00:00:00Z","timestamp":1175040000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2007,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>In rheumatoid arthritis (RA), formation of new blood vessels is necessary to meet the nutritional and oxygen requirements of actively proliferating synovial tissue. The aim of this study was to analyze the specific synovial vascular expression profiles of several angiogenesis\u2010related genes as well as CD82 in RA compared with osteoarthritis (OA), using laser\u2010mediated microdissection (LMM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>LMM and subsequent real\u2010time polymerase chain reaction were used in combination with immunohistochemical analysis for area\u2010specific analysis of messenger RNA (mRNA) and protein expression of vascular endothelial growth factor (VEGF), VEGF receptor 1 (VEGFR\u20101), VEGFR\u20102, hypoxia\u2010inducible factor 1\u03b1 (HIF\u20101\u03b1), HIF\u20102\u03b1, platelet\u2010derived growth factor receptor \u03b1 (PDGFR\u03b1), PDGFR\u03b2, inhibitor of DNA binding\/differentiation 2 (Id2), and CD82 in RA and OA synovial microvasculature and synovial lining.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Expression of Id2 mRNA was significantly lower in RA synovial vessels compared with OA synovial vessels (<jats:italic>P<\/jats:italic> = 0.0011), whereas expression of VEGFR\u20101 was significantly higher in RA (<jats:italic>P<\/jats:italic> = 0.0433). No differences were observed for the other parameters. At the protein level, no statistically significant differences were observed for any parameter, although Id2 levels were 2.5\u2010fold lower in RA (<jats:italic>P<\/jats:italic> = 0.0952). However, the number of synovial blood vessels and the number of VEGFR\u20102\u2013expressing blood vessels were significantly higher in RA compared with OA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results underscore the importance of area\u2010specific gene expression analysis in studying the pathogenesis of RA and support LMM as a robust tool for this purpose. Of note, our results indicate that previously described differences between RA and OA in the expression of angiogenic molecules are attributable to higher total numbers of synovial and vascular cells expressing these molecules in RA rather than higher expression levels in the individual cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.22450","type":"journal-article","created":{"date-parts":[[2007,3,28]],"date-time":"2007-03-28T18:18:11Z","timestamp":1175105891000},"page":"1094-1105","source":"Crossref","is-referenced-by-count":20,"title":["Analysis of vascular gene expression in arthritic synovium by laser\u2010mediated microdissection"],"prefix":"10.1002","volume":"56","author":[{"given":"Atsushi","family":"Hashimoto","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ingo H.","family":"Tarner","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rainer 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