{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T14:31:42Z","timestamp":1786977102880,"version":"build-2736575974"},"reference-count":46,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,6,28]],"date-time":"2007-06-28T00:00:00Z","timestamp":1182988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>The cartilage proteins melanoma inhibitory activity (MIA) and human cartilage gp\u201039 (HC gp\u201039) are candidate autoantigens in rheumatoid arthritis (RA). The present study was undertaken to investigate the endogenous HLA\u2013DR4\u2013restricted presentation of these self proteins, in order to seek in vivo evidence in support of their potential immunologic role.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>MIA and HC gp\u201039 were assessed in synovial fluid (SF) by enzyme\u2010linked immunosorbent assay and in synovial tissue (ST) by immunohistochemistry. Presentation by SF cells was investigated using specific, HLA\u2013DR\u2013restricted T cell hybridomas.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>MIA and HC gp\u201039 were detected in RA SF and ST, as well as in specimens from patients with other forms of arthritis. When HC gp\u201039\u2013specific and MIA\u2010specific HLA\u2013DR4\u2013restricted T cell hybridomas raised in HLA\u2013DR4\u2013transgenic mice were incubated with RA SF cells as antigen\u2010presenting cells in the presence of HC gp\u201039 or MIA peptides, the corresponding T cell hybridomas showed strong responses, which were blocked by anti\u2013HLA\u2013DR antibodies. Weaker but qualitatively similar responses were observed with exogenous protein, indicating uptake and processing of these antigens by SF cells. More importantly, without addition of peptide or protein, endogenous presentation of MIA and HC gp\u201039 was detected in SF cells from 53% and 80% of HLA\u2013DRB1*0401\u2013positive RA patients, respectively. In addition, SF cells from 3 of 10 patients with spondylarthritis exhibited endogenous HC gp\u201039 presentation.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>These data indicate that immunodominant epitopes of MIA and HC gp\u201039 are actively presented in an HLA\u2013DR\u2013restricted manner in the inflamed RA joint. The question remains as to whether this leads to activation of autoreactive T cells, which could play a role in either the immunopathology or the immunomodulation of arthritis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.22651","type":"journal-article","created":{"date-parts":[[2007,6,28]],"date-time":"2007-06-28T13:48:16Z","timestamp":1183038496000},"page":"2150-2159","source":"Crossref","is-referenced-by-count":22,"title":["Endogenous HLA\u2013DR\u2013restricted presentation of the cartilage antigens human cartilage gp\u201039 and melanoma inhibitory activity in the inflamed rheumatoid joint"],"prefix":"10.1002","volume":"56","author":[{"given":"M. J. 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