{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T19:00:30Z","timestamp":1790622030674,"version":"4.1.0"},"reference-count":17,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2007,6,28]],"date-time":"2007-06-28T00:00:00Z","timestamp":1182988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2007,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To assess whether the recently discovered exosome\u2010associated proteins MPP6, C1D, KIAA0052\/hMtr4, hSki2, and hSki8 are targeted by autoantibodies, and to determine whether these autoantibodies are accompanied by antibodies directed to the established exosome\u2010associated autoantigens PM\u2013Scl\u201075 and PM\u2013Scl\u2010100.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Complementary DNAs encoding the recently identified human exosome\u2013associated proteins were expressed as His\u2010tagged fusion proteins in <jats:italic>Escherichia coli<\/jats:italic> cells. Sera obtained from patients with several different autoimmune diseases were analyzed for the presence of autoantibodies directed to these proteins, in an enzyme\u2010linked immunosorbent assay (ELISA). The ELISA data obtained for C1D were confirmed by Western blot analysis, using recombinant C1D.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All exosome\u2010associated proteins were found to be targeted by autoantibodies, although the frequency with which such antibodies occurred in patient sera was relatively low, with the exception of anti\u2010C1D antibodies. Autoantibodies recognizing C1D were detected in 7 of 30 patients (23%) with the polymyositis (PM)\u2013scleroderma overlap syndrome; this frequency was similar to the frequencies for the established autoantigens PM\u2013Scl\u201075c (27%) and PM\u2013Scl\u2010100 (23%). Importantly, several patients with the PM\u2013scleroderma overlap syndrome had anti\u2010C1D antibodies but no anti\u2013PM\u2010Scl antibodies. Anti\u2010C1D autoantibodies were observed in only 2 of 204 patients with other diseases, including PM, dermatomyositis, and scleroderma.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our results demonstrate that the recently identified exosome\u2010associated protein C1D is a major autoantigen in patients with the PM\u2013scleroderma overlap syndrome and suggest that the use of recombinant C1D as an autoantibody target may aid in diagnosis of the PM\u2013scleroderma overlap syndrome.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.22710","type":"journal-article","created":{"date-parts":[[2007,6,28]],"date-time":"2007-06-28T18:17:34Z","timestamp":1183054654000},"page":"2449-2454","source":"Crossref","is-referenced-by-count":16,"title":["C1D is a major autoantibody target in patients with the polymyositis\u2013scleroderma overlap syndrome"],"prefix":"10.1002","volume":"56","author":[{"given":"Geurt","family":"Schilders","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wilma Vree","family":"Egberts","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Reinout","family":"Raijmakers","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ger J. M.","family":"Pruijn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,6,28]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1080\/08916930600622132"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0074-7696(06)51005-8"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.173.4.941"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.176.4.973"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1007\/s001099900072"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1729"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20056"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(01)00578-5"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar389"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/17.4.1128"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.23.19.6982-6992.2003"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gki982"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1292105"},{"key":"e_1_2_7_15_2","first-page":"20","volume-title":"Primer on the rheumatic diseases","author":"Cronin ME","year":"1988"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0022-1759(98)00170-7"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar2119"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.6.815"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.22710","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.22710","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T04:07:35Z","timestamp":1698725255000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.22710"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,6,28]]},"references-count":17,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2007,7]]}},"alternative-id":["10.1002\/art.22710"],"URL":"https:\/\/doi.org\/10.1002\/art.22710","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,6,28]]}}}