{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:28:22Z","timestamp":1791300502159,"version":"4.1.0"},"reference-count":16,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2007,8,30]],"date-time":"2007-08-30T00:00:00Z","timestamp":1188432000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2007,9]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Myositis\u2010specific autoantibodies (MSAs) are directed against cell machinery proteins such as aminoacyl\u2013transfer RNA synthetases, signal recognition particle, Mi\u20102, and CADM\u2010140. Because serologic subsets can define patients with specific clinical manifestations, the identification of further MSAs may help to identify additional disease subsets within the myositis spectrum.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sera from 20 adult patients with dermatomyositis (DM) were screened for autoantibodies. Two patients were further characterized due to the presence of the same novel immunoprecipitation (IP) pattern on sodium docecyl sulfate\u2013polyacrylamide gel electrophoresis (SDS\u2010PAGE) and similar clinical manifestations. Both patients presented with cutaneous disease, followed by proximal myositis 6 months later. Both patients had associated nonspecific interstitial pneumonia but no signs of malignancy. The novel targets were identified using a combination of IP, SDS\u2010PAGE, and matrix\u2010assisted laser desorption ionization\u2013time\u2010of\u2010flight mass spectrometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Indirect HEp\u20102 immunofluorescence on sera from both patients displayed a diffuse, coarse, speckled, nucleolar\u2010sparing pattern. IP revealed the presence of previously uncharacterized bands at \u223c40 kd and \u223c90 kd in both patients. The novel targets were identified as the small ubiquitin\u2010like modifier 1 (SUMO\u20101) activating enzyme A subunit and SUMO\u20101 activating enzyme B subunit.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings reveal previously uncharacterized autoantibodies directed against a protein involved in posttranslational modification, the SUMO activating enzyme, in 2 patients with DM who had similar clinical features, including severe skin disease and interstitial pneumonia.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.22862","type":"journal-article","created":{"date-parts":[[2007,8,30]],"date-time":"2007-08-30T19:17:29Z","timestamp":1188501449000},"page":"3132-3137","source":"Crossref","is-referenced-by-count":141,"title":["Identification of a novel autoantibody directed against small ubiquitin\u2010like modifier activating enzyme in dermatomyositis"],"prefix":"10.1002","volume":"56","author":[{"given":"Zo\u00eb","family":"Betteridge","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harsha","family":"Gunawardena","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean","family":"North","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jenna","family":"Slinn","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Neil","family":"McHugh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2007,8,30]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-199111000-00002"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-996-0025-3"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.09.021"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0854.2002.30601.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502202920807"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230510"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251101"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kem045"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1992.tb01940.x"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1399-0039.1993.tb01991.x"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200003)43:3<599::AID-ANR17>3.0.CO;2-T"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/hep.20619"},{"key":"e_1_2_7_15_2","first-page":"581","article-title":"Dermatopolymyositis and primary biliary cirrhosis: a rare association","volume":"25","author":"Rain F","year":"1996","journal-title":"Presse Med"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.274.15.10618"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-5793(99)00367-1"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.22862","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.22862","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T09:45:50Z","timestamp":1697017550000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.22862"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2007,8,30]]},"references-count":16,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2007,9]]}},"alternative-id":["10.1002\/art.22862"],"URL":"https:\/\/doi.org\/10.1002\/art.22862","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2007,8,30]]}}}