{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,23]],"date-time":"2026-02-23T14:37:27Z","timestamp":1771857447864,"version":"3.50.1"},"reference-count":52,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2007,11,29]],"date-time":"2007-11-29T00:00:00Z","timestamp":1196294400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2007,12,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine spontaneous and activation\u2010induced apoptosis of peripheral blood lymphocytes (PBLs) from patients with active untreated adult\u2010onset Still's disease (AOSD) and to examine the role of interleukin\u201018 (IL\u201018) involved in the apoptosis related to this disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The percentages of spontaneous and IL\u201018\u2013stimulated apoptotic lymphocytes in peripheral blood of 20 patients with active untreated AOSD, 20 with active untreated systemic lupus erythematosus (SLE), and 20 healthy controls were determined using annexin V\/propidium iodide staining and flow cytometry. Serum IL\u201018 levels were measured using enzyme\u2010linked immunosorbent assay. The transcripts of caspase 3 gene and apoptosis\u2010regulating genes, including Fas, FasL, Bcl\u20102, and p53 in IL\u201018\u2013treated peripheral blood mononuclear cells (PBMCs) from 8 AOSD patients, 4 SLE patients, and 4 healthy controls, were examined by real\u2010time quantitative polymerase chain reaction.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Significantly higher percentages of spontaneous and IL\u201018\u2013stimulated apoptotic PBLs were found in patients with active untreated AOSD and those with active untreated SLE than in healthy controls. The percentages of spontaneous and IL\u201018\u2013stimulated apoptotic lymphocytes correlated positively with clinical activity scores and serum IL\u201018 levels for AOSD patients and SLE patients. The percentages of spontaneous and activation\u2010induced apoptotic PBLs significantly declined, paralleling clinical remission and the decrease in serum IL\u201018 levels after effective therapy in AOSD patients. Up\u2010regulation of FasL and p53 transcripts was demonstrated in IL\u201018\u2013treated PBMCs from AOSD patients and SLE patients in a dose\u2010dependent manner.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The increased apoptosis of PBLs from AOSD patients may be associated with the effect of IL\u201018 through up\u2010regulation of FasL and p53 transcripts.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.23088","type":"journal-article","created":{"date-parts":[[2007,11,29]],"date-time":"2007-11-29T17:19:03Z","timestamp":1196356743000},"page":"1530-1538","source":"Crossref","is-referenced-by-count":36,"title":["Increased apoptosis of peripheral blood lymphocytes and its association with interleukin\u201018 in patients with active untreated adult\u2010onset Still's 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