{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T02:06:27Z","timestamp":1791252387373,"version":"4.1.0"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2008,3,27]],"date-time":"2008-03-27T00:00:00Z","timestamp":1206576000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2008,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The detection of high titers of antibodies against small nuclear ribonucleoproteins (snRNP) is a diagnostic finding in patients in whom systemic lupus erythematosus (SLE) is suspected. Endogenous RNA molecules within snRNP trigger Toll\u2010like receptor 7 (TLR\u20107) activation in B cells and dendritic cells, leading to anti\u2010snRNP antibody production, which is associated with the development of immune complex nephritis in SLE. The purpose of this study was to investigate the role of TLR\u20107 in anti\u2010snRNP antibody production and renal disease in SLE induced by an exogenous factor in the absence of genetic predisposition, using the pristane\u2010induced murine lupus model.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Serum autoantibodies, IgG isotypes, and cytokine levels in pristane\u2010treated wild\u2010type and TLR\u20107\u2013deficient mice were analyzed by enzyme\u2010linked immunosorbent assay. Histopathologic changes in mouse kidneys were determined by light immunofluorescence microscopy. Cell subsets in splenocytes and peritoneal lavage cells from the mice were examined by flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that anti\u2010snRNP antibody production induced by pristane treatment was entirely dependent on the expression of TLR\u20107, whereas anti\u2013double\u2010stranded DNA antibody production was not affected by a lack of TLR\u20107. Impaired anti\u2010snRNP antibody production in TLR\u20107\u2013deficient mice was paralleled by lower levels of glomerular IgG and complement deposits, as well as less severe glomerulonephritis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>TLR\u20107 is specifically required for the production of RNA\u2010reactive autoantibodies and the development of glomerulonephritis in pristane\u2010induced murine lupus, a model of environmentally triggered SLE in the absence of genetic susceptibility to autoimmunity. Specific interference with TLR\u20107 activation by endogenous TLR\u20107 ligands may therefore be a promising novel strategy for the treatment of SLE.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.23407","type":"journal-article","created":{"date-parts":[[2008,3,28]],"date-time":"2008-03-28T02:20:20Z","timestamp":1206670820000},"page":"1107-1115","source":"Crossref","is-referenced-by-count":126,"title":["Requirement of toll\u2010like receptor 7 for pristane\u2010induced production of autoantibodies and development of murine lupus nephritis"],"prefix":"10.1002","volume":"58","author":[{"given":"Emina","family":"Savarese","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Steinberg","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rahul D.","family":"Pawar","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wolfgang","family":"Reindl","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shizuo","family":"Akira","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hans\u2010Joachim","family":"Anders","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anne","family":"Krug","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2008,3,27]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021933"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1957"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.11.6491"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050630"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1038\/416603a"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00323-6"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20031942"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI23025"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2005-07-2650"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20051696"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.171.6.3296"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.7.4043"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1124978"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0603912103"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.07.013"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.07.014"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2006.050125"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.7.4777"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.92.24.10934"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni758"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1681\/ASN.2006101162"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1038\/ki.1984.75"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2004.06.007"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.smim.2006.12.005"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21031"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052228"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20052438"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1093\/ndt\/gfl336"}],"container-title":["Arthritis &amp; 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