{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T13:01:44Z","timestamp":1775221304018,"version":"3.50.1"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2008,5,31]],"date-time":"2008-05-31T00:00:00Z","timestamp":1212192000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2008,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine gene expression of the interleukin\u201017 (IL\u201017) family members (IL\u201017A\u2013F) in rheumatoid subcutaneous nodules, and to assess the cytokines involved in regulating IL\u201017A expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Total RNA was isolated from 19 nodules obtained from 16 different patients with rheumatoid arthritis (RA). Reverse transcription\u2013polymerase chain reaction (PCR) was used to screen for gene expression of the IL\u201017 subtypes (IL\u201017A\u2013F) in all nodules. Quantitative real\u2010time PCR was used to measure the expression of interferon\u2010\u03b3 (IFN\u03b3), IL\u20106, IL\u201023, IL\u201012, and transforming growth factor \u03b2 (TGF\u03b2), relative to GAPDH as control, in a subset of 10 nodules.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>IL\u201017A gene expression was present in only 1 of 19 nodules, IL\u201017B in 17 of 19 nodules, IL\u201017C in 18 of 19 nodules, IL\u201017D in 16 of 19 nodules, and IL\u201017E in 3 of 19 nodules. IL\u201017F was absent in all samples. Cytokines that stimulate IL\u201017A production (IL\u20106, IL\u201023) as well as those that inhibit IL\u201017A production (IL\u201012, IFN\u03b3, TGF\u03b2) were present in the majority of nodules. Quantitative real\u2010time PCR showed a similar pattern of gene expression for the individual cytokines between the different nodules. The mean \u00b1 SD expression of IL\u20106 relative to GAPDH was 2.28 \u00b1 2.2 ng, and that of TGF\u03b2 was 2.96 \u00b1 1.14 ng. There was a lower relative expression of IL\u201023 (0.05 \u00b1 0.05 ng), while the expression of IFN\u03b3 was 0.67 \u00b1 0.68 ng and that of IL\u201012 was 0.48 \u00b1 0.23 ng.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>IL\u201017 family members are varyingly expressed in rheumatoid nodules. The paucity of IL\u201017A in nodules suggests an important difference from that observed in the synovium. The expression of IL\u201023 below a critical threshold level seems the most likely explanation for the virtual absence of IL\u201017A. The presence of tissue destruction within the nodule despite the absence of IL\u201017A suggests that IL\u201017A may be an important amplifier rather than an absolute requirement for inflammation in RA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.23455","type":"journal-article","created":{"date-parts":[[2008,5,31]],"date-time":"2008-05-31T21:18:07Z","timestamp":1212268687000},"page":"1601-1608","source":"Crossref","is-referenced-by-count":32,"title":["Different T cell subsets in the nodule and synovial membrane: Absence of interleukin\u201017A in rheumatoid nodules"],"prefix":"10.1002","volume":"58","author":[{"given":"Lisa 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