{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,12]],"date-time":"2026-06-12T22:35:32Z","timestamp":1781303732443,"version":"3.54.1"},"reference-count":27,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T00:00:00Z","timestamp":1233187200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2009,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To explore the role of immune cells in anti\u2010RNP autoimmunity in a murine model of pneumonitis or glomerulonephritis, using adoptive transfer techniques.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Donor mice were immunized with 50 \u03bcg of U1\u201370\u2010kd small nuclear RNP fusion protein and 50 \u03bcg of U1 RNA adjuvant. Whole splenocytes as well as CD4+ cell and dendritic cell (DC) subsets from the immunized mice were infused into naive syngeneic recipients. Anti\u2010RNP and T cell responses were assessed by immunoblotting, enzyme\u2010linked immunosorbent assay, and flow cytometry. Development of renal or lung disease was assessed by histology and urinalysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Unfractionated splenocytes from donor mice without proteinuria induced predominantly lung disease in recipients (8 [57%] of 14 versus 2 [14%] of 14 developing renal disease; <jats:italic>P<\/jats:italic> = 0.046). However, infusion of CD4+ cells from donors without proteinuria induced renal disease more frequently than lung disease (7 [70%] of 10 versus 2 [20%] of 10; <jats:italic>P<\/jats:italic> = 0.01); adoptive transfer of RNP+CD4+ T cells from short\u2010term culture yielded similar results (renal disease in 8 [73%] of 11 recipients versus lung disease in 3 [27%] of 11). Cotransfer of splenic myeloid DCs and CD4+ T cells from immunized donors prevented induction of renal disease in all 5 recipients (<jats:italic>P<\/jats:italic> = 0.026 versus recipients of fresh CD4+ cells alone), although lung disease was still observed in 1 of 5 mice. Transfer of myeloid DCs alone from immunized donors induced lung disease in 3 (60%) of 5 recipients, without evidence of nephritis. Cotransfer of splenocytes from mice with and those without nephritis led to renal disease in 4 of 5 recipients, without evidence of lung disease.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings indicate that RNP+CD4+ T cells are sufficient to induce anti\u2010RNP autoimmunity, tissue targeting in anti\u2010RNP autoimmunity can be deviated to either a renal or pulmonary phenotype depending on the presence of accessory cells such as myeloid DCs, and DC subsets can play a role in both propagation of autoimmunity and end\u2010organ targeting.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.24256","type":"journal-article","created":{"date-parts":[[2009,1,29]],"date-time":"2009-01-29T06:30:47Z","timestamp":1233210647000},"page":"534-542","source":"Crossref","is-referenced-by-count":15,"title":["Tissue targeting of anti\u2010RNP autoimmunity: Effects of T cells and myeloid dendritic cells in a murine model"],"prefix":"10.1002","volume":"60","author":[{"given":"Eric L.","family":"Greidinger","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"YunJuan","family":"Zang","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Irina","family":"Fernandez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariana","family":"Berho","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mehdi","family":"Nassiri","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Laisel","family":"Martinez","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert W.","family":"Hoffman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,1,29]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197611182952101"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa021933"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200102)44:2<368::AID-ANR55>3.0.CO;2-6"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21566"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1080\/08916930701262986"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.24.12010"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1006\/jaut.2002.0601"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.12.8444"},{"key":"e_1_2_6_10_2","doi-asserted-by":"crossref","first-page":"6460","DOI":"10.4049\/jimmunol.151.11.6460","article-title":"Human T cell clones reactive against U\u2010small nuclear ribonucleoprotein autoantigens from connective tissue disease patients and healthy individuals","volume":"151","author":"Hoffman RW","year":"1993","journal-title":"J Immunol"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780400818"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20287"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.169.6.3429"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22601"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.172.1.709"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200323797"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.08.010"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh575"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.6.3368"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.3.1937"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1164\/rccm.200701-054OC"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2005-0382OC"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.2.1080"},{"key":"e_1_2_6_24_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2007.05.030"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2006.07.013"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199805)41:5<768::AID-ART3>3.0.CO;2-Z"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199901)42:1<190::AID-ANR29>3.0.CO;2-J"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199901)42:1<191::AID-ANR30>3.0.CO;2-7"}],"container-title":["Arthritis &amp; 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