{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T22:52:13Z","timestamp":1790895133713,"version":"4.1.0"},"reference-count":30,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,3,30]],"date-time":"2009-03-30T00:00:00Z","timestamp":1238371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Sj\u00f6gren's syndrome (SS) is characterized by atrophy and malfunction of the acinar cells. The aim of this study was to investigate whether type IV collagen \u03b1\u2010chain composition of acinar cell compartments could be abnormal in diseased glands.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Messenger RNA (mRNA) from human submandibular gland (HSG) cells, cultured with or without growth factor\u2013depleted Matrigel, was analyzed using quantitative reverse transcription\u2013polymerase chain reaction (RT\u2010PCR). Labial salivary glands were analyzed using quantitative RT\u2010PCR and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>HSG cells of both the ductal and acinar phenotypes synthesized all \u03b1\u2010chain mRNA, in particular those of the \u03b11 and \u03b12 chains. Labial salivary glands (LSGs) contained \u03b11\/2 chains but also contained mRNA of all the other \u03b1\u2010chains, although the mRNA copy numbers for the \u03b13 and \u03b14 chains were low, and the corresponding proteins were absent. Type IV collagen \u03b11\/2\u2010chains were observed in all tubuloalveolar basement membranes. In healthy glands, \u03b15 and \u03b16 chains were continuous around ducts but discontinuous around acini. In SS glands, these chains were absent or patchy around the ducts and absent around the acini.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Ductal and acinar epithelial cells are able to locally produce mRNA for all 6 different \u03b1\u2010chains. Type IV collagen \u03b11\/2\u2010chains seem to form the backbone in the tubuloalveolar basement membrane in salivary glands. Type IV collagen \u03b13 and \u03b14 chain mRNA were found in cultured salivary epithelial cells and LSG explants but were not translated to the corresponding \u03b1\u2010chains in LSGs. Both \u03b15 and \u03b16 mRNA were observed in salivary epithelial cells and glands. In healthy glands, immunolabeling always disclosed corresponding \u03b1\u2010chains around ducts, but their synthesis and\/or degradation seemed to be locally regulated around acinar cells.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.24388","type":"journal-article","created":{"date-parts":[[2009,3,31]],"date-time":"2009-03-31T01:45:08Z","timestamp":1238463908000},"page":"938-945","source":"Crossref","is-referenced-by-count":10,"title":["Abnormal basement membrane type IV collagen \u03b1\u2010chain composition in labial salivary glands in Sj\u00f6gren's syndrome"],"prefix":"10.1002","volume":"60","author":[{"given":"P.","family":"Poduval","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T.","family":"Sillat","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Virtanen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"P.","family":"Porola","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Y. 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