{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T10:39:50Z","timestamp":1790419190053,"version":"4.1.0"},"reference-count":49,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,3,30]],"date-time":"2009-03-30T00:00:00Z","timestamp":1238371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2009,4,15]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine the presence of small integrin\u2010binding ligand <jats:italic>N<\/jats:italic>\u2010linked glycoprotein (SIBLING) and bone components in juvenile dermatomyositis (DM) pathologic calcifications.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Calcifications were removed from 4 girls with juvenile DM symptoms for mean \u00b1 SD 36.9 \u00b1 48.3 months and were stained for SIBLING proteins: full\u2010length osteopontin (OPN), bone sialoprotein (BSP), dentin matrix protein 1 (DMP1), dentin phosphoprotein (DPP), and matrix extracellular phosphoglycoprotein (MEPE); bone markers: osteocalcin (OC), core\u2010binding factor \u03b1 1 (CBF\u03b11), and alkaline phosphatase (AP) for osteoblasts; tartrate\u2010resistant acid phosphatase (TRAP) for osteoclasts; and the mineral regulators osteonectin (ON) and matrix Gla protein (MGP). The deposit center, periphery, adjacent connective tissue, and vascular endothelial cells were examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Alizarin red stained calcified deposits that did not localize with collagen, like bone, under polarized light. Hematoxylin and eosin stain revealed a paucity of connective tissue and absence of bone\u2010like structures. The deposits, connective tissue, and vascular endothelial cells were positive for BSP, DPP, DMP1, and AP; MEPE was not detected. OC, ON, and MGP were present in the deposits and vascular endothelial cells; OPN and CBF\u03b11 were present in deposits and connective tissue. TRAP\u2010positive osteoclasts were localized to the calcification periphery.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The disorganized juvenile DM calcifications differ in structure, composition, and protein content from bone, suggesting that they may not form through an osteogenic pathway. Osteoclasts at the deposit surface represent an attempt to initiate its resolution.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.24391","type":"journal-article","created":{"date-parts":[[2009,3,31]],"date-time":"2009-03-31T01:47:56Z","timestamp":1238464076000},"page":"501-508","source":"Crossref","is-referenced-by-count":31,"title":["Juvenile dermatomyositis calcifications selectively displayed markers of bone formation"],"prefix":"10.1002","volume":"61","author":[{"given":"Annette L.","family":"Urganus","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong\u2010dong","family":"Zhao","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren 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