{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T18:08:42Z","timestamp":1791223722751,"version":"4.1.0"},"reference-count":34,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2009,3,30]],"date-time":"2009-03-30T00:00:00Z","timestamp":1238371200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2009,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>A role of microRNA, which are \u223c22\u2010nucleotide noncoding RNAs, has recently been recognized in human diseases. The objective of this study was to identify the expression pattern of microRNA\u2010146a (miR\u2010146a) in cartilage from patients with osteoarthritis (OA).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>The expression of miR\u2010146a in cartilage from 15 patients with OA was analyzed by quantitative reverse transcription\u2013polymerase chain reaction (RT\u2010PCR) and by in situ hybridization. Induction of the expression of miR\u2010146a by cultures of normal human articular chondrocytes following stimulation with interleukin\u20101\u03b2 (IL\u20101\u03b2) was examined by quantitative RT\u2010PCR.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      All cartilage samples were divided into 3 groups according to a modification of the Mankin score (grade I = mild OA scored 0\u20135, grade II = moderate OA scored 6\u201310, and grade III = severe OA scored 11\u201314). In grade I OA cartilage samples, the expression of miR\u2010146a and COL2A1 was significantly higher than that in the other groups (\n                      <jats:italic>P<\/jats:italic>\n                      &lt; 0.05). In grades II and III OA cartilage, the expression of miR\u2010146a and COL2A1 was decreased, whereas the expression of matrix metalloproteinase 13 (MMP\u201013) was elevated in grade II OA cartilage. These data showed that miR\u2010146a is expressed intensely in cartilage with a low Mankin grade and that miR\u2010146a expression decreases in parallel with the level of MMP\u201013 expression. Tissue section in situ hybridization of primary miR\u2010146a (pri\u2010miR\u2010146a) revealed that pri\u2010miR\u2010146a was expressed in chondrocytes residing in all tissue layers, especially in the superficial layer, where it was intensely expressed. The expression of miR\u2010146 was markedly elevated by IL\u20101\u03b2 stimulation in human chondrocytes in vitro.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study shows that miR\u2010146 is intensely expressed in low\u2010grade OA cartilage and that its expression is induced by stimulation of IL\u20101\u03b2. Thus, miR\u2010146 might play a role in OA cartilage pathogenesis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.24404","type":"journal-article","created":{"date-parts":[[2009,3,30]],"date-time":"2009-03-30T21:45:08Z","timestamp":1238449508000},"page":"1035-1041","source":"Crossref","is-referenced-by-count":293,"title":["Expression of MicroRNA\u2010146a in osteoarthritis cartilage"],"prefix":"10.1002","volume":"60","author":[{"given":"Keiichiro","family":"Yamasaki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomoyuki","family":"Nakasa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shigeru","family":"Miyaki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masakazu","family":"Ishikawa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Deie","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuo","family":"Adachi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuji","family":"Yasunaga","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Asahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mitsuo","family":"Ochi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,3,30]]},"reference":[{"key":"e_1_2_6_2_2","first-page":"818","article-title":"Cartilage hypertrophy following canine anterior cruciate ligament transaction differs among different areas of the joint","volume":"16","author":"Adams ME","year":"1989","journal-title":"J Rheumatol"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/jor.1100100406"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780260708"},{"key":"e_1_2_6_5_2","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1302\/0301-620X.59B1.576611","article-title":"An experimental model of osteoarthritis; 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