{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T04:53:25Z","timestamp":1791176005871,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2009,4,29]],"date-time":"2009-04-29T00:00:00Z","timestamp":1240963200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2009,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Wegener's granulomatosis (WG) is strongly associated with antineutrophil cytoplasmic autoantibodies (ANCAs) directed against proteinase 3 (PR3). Recent studies have shown that membrane\u2010bound PR3 (mPR3) is differentially expressed and colocalizes with CD177\/NB1 on circulating neutrophils. We undertook this study to assess the differential expression of CD177 on neutrophils from patients with ANCA\u2010associated systemic vasculitis (ASV) in comparison with patients with systemic lupus erythematosus (SLE), patients with rheumatoid arthritis (RA), and healthy individuals, and to investigate whether colocalization of mPR3 and CD177 affects anti\u2010PR3\u2013mediated neutrophil activation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Expression of CD177 and mPR3 was analyzed by flow cytometry on isolated neutrophils from patients with ASV (n = 53), those with SLE (n = 30), those with RA (n = 26), and healthy controls (n = 31). Neutrophil activation mediated by anti\u2010PR3 antibodies was assessed by measuring the oxidative burst with a dihydrorhodamine assay.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Percentages of CD177\u2010expressing neutrophils were significantly higher in patients with ASV and those with SLE than in healthy controls. In 3 healthy donors, CD177 expression was not detected. After priming with tumor necrosis factor \u03b1, neutrophils remained negative for CD177 while mPR3 expression was induced. Neutrophils from CD177\u2010negative donors or CD177\u2212 neutrophils sorted from donors with bimodal expression were susceptible to anti\u2010PR3\u2013mediated oxidative burst. Variation in the extent of anti\u2010PR3\u2013mediated neutrophil activation among different donors occurred independent of the percentage of CD177\u2010expressing neutrophils.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Membrane expression of CD177 on circulating neutrophils is increased in patients with ASV and in those with SLE, but not in RA patients. However, primed neutrophils from CD177\u2010negative individuals also express mPR3 and are susceptible to anti\u2010PR3\u2013mediated oxidative burst, suggesting that recruitment of CD177\u2010independent mPR3 is involved in anti\u2010PR3\u2013induced neutrophil activation.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.24442","type":"journal-article","created":{"date-parts":[[2009,4,29]],"date-time":"2009-04-29T10:52:59Z","timestamp":1241002379000},"page":"1548-1557","source":"Crossref","is-referenced-by-count":87,"title":["Coexpression of CD177 and membrane proteinase 3 on neutrophils in antineutrophil cytoplasmic autoantibody\u2013associated systemic vasculitis: Anti\u2013proteinase 3\u2013mediated neutrophil activation is independent of the role of CD177\u2010expressing neutrophils"],"prefix":"10.1002","volume":"60","author":[{"given":"N.","family":"Hu","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J.","family":"Westra","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. 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