{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T01:28:50Z","timestamp":1779240530601,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T00:00:00Z","timestamp":1246233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To determine whether peripheral blood mononuclear cells (PBMCs) from children with recent\u2010onset polyarticular juvenile idiopathic arthritis (JIA) exhibit biologically or clinically informative gene expression signatures.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood samples were obtained from 59 healthy children and 61 children with polyarticular JIA prior to treatment with second\u2010line medications, such as methotrexate or biologic agents. RNA was extracted from isolated mononuclear cells, fluorescence labeled, and hybridized to commercial gene expression microarrays (Affymetrix HG\u2010U133 Plus 2.0). Data were analyzed using analysis of variance at a 5% false discovery rate threshold after robust multichip analysis preprocessing and distance\u2010weighted discrimination normalization.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Initial analysis revealed 873 probe sets for genes that were differentially expressed between polyarticular JIA patients and healthy controls. Hierarchical clustering of these probe sets distinguished 3 subgroups within the polyarticular JIA group. Prototypical patients within each subgroup were identified and used to define subgroup\u2010specific gene expression signatures. One of these signatures was associated with monocyte markers, another with transforming growth factor \u03b2\u2013inducible genes, and a third with immediate early genes. Correlation of gene expression signatures with clinical and biologic features of JIA subgroups suggested relevance to aspects of disease activity and supported the division of polyarticular JIA into distinct subsets.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Gene expression signatures in PBMCs from patients with recent\u2010onset polyarticular JIA reflect discrete disease processes and offer a molecular classification of disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.24534","type":"journal-article","created":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T15:05:41Z","timestamp":1246287941000},"page":"2113-2123","source":"Crossref","is-referenced-by-count":62,"title":["Gene expression signatures in polyarticular juvenile idiopathic arthritis demonstrate disease heterogeneity and offer a molecular classification of disease subsets"],"prefix":"10.1002","volume":"60","author":[{"given":"Thomas A.","family":"Griffin","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Michael G.","family":"Barnes","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Norman T.","family":"Ilowite","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Judyann C.","family":"Olson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David D.","family":"Sherry","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beth S.","family":"Gottlieb","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bruce J.","family":"Aronow","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Paul","family":"Pavlidis","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claas H.","family":"Hinze","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sherry","family":"Thornton","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Susan D.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexei A.","family":"Grom","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Robert A.","family":"Colbert","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"David N.","family":"Glass","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2009,6,29]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"1991","article-title":"Revision of the proposed classification criteria for juvenile idiopathic arthritis: Durban, 1997","volume":"25","author":"Petty RE","year":"1998","journal-title":"J Rheumatol"},{"key":"e_1_2_7_3_2","first-page":"390","article-title":"International League of Associations for Rheumatology classification of juvenile idiopathic arthritis: second revision, Edmonton, 2001","volume":"31","author":"Petty RE","year":"2004","journal-title":"J Rheumatol"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(07)60363-8"},{"key":"e_1_2_7_5_2","first-page":"72","article-title":"Update on treatment of arthritis in children: new treatments, new goals","volume":"64","author":"Lovell DJ","year":"2006","journal-title":"Bull NYU Hosp Jt Dis"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh224"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22981"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22644"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1018"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070070"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24601"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1093\/biostatistics\/4.2.249"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btg385"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/sim.4780090710"},{"key":"e_1_2_7_15_2","first-page":"492","article-title":"Measurement of biomarkers in juvenile idiopathic arthritis patients and their significant association with disease severity: a comparative study","volume":"26","author":"Gilliam BE","year":"2008","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1532\/LH96.03056"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.0207117"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.11.6037"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh583"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.174.9.5837"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2004.03.018"},{"key":"e_1_2_7_22_2","doi-asserted-by":"crossref","first-page":"13809","DOI":"10.1016\/S0021-9258(18)77420-6","article-title":"\u03b1\u2010 and \u03b2\u2010 adrenergic stimulation induces distinct patterns of immediate early gene expression in neonatal rat myocardial cells. fos\/jun expression is associated with sarcomere assembly; Egr\u20101 induction is primarily an \u03b11\u2010mediated response","volume":"265","author":"Iwaki K","year":"1990","journal-title":"J Biol Chem"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.6.2979"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10972"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1038\/ncb731"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/18.5.1280"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.91.26.12599"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.07709.x"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.embor.7400501"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2249.1997.4331330.x"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1996.tb08288.x"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20061531"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20945"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.24534","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.24534","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T06:40:27Z","timestamp":1698734427000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.24534"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,6,29]]},"references-count":32,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2009,7]]}},"alternative-id":["10.1002\/art.24534"],"URL":"https:\/\/doi.org\/10.1002\/art.24534","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,6,29]]}}}