{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T01:27:29Z","timestamp":1790126849364,"version":"4.0.1"},"reference-count":37,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T00:00:00Z","timestamp":1246233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To identify differences in peripheral blood gene expression between patients with different subclasses of juvenile idiopathic arthritis (JIA) and healthy controls in a multicenter study of patients with recent\u2010onset JIA prior to treatment with disease\u2010modifying antirheumatic drugs (DMARDs) or biologic agents.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Peripheral blood mononuclear cells (PBMCs) from 59 healthy children and 136 patients with JIA (28 with enthesitis\u2010related arthritis [ERA], 42 with persistent oligoarthritis, 45 with rheumatoid factor [RF]\u2013negative polyarthritis, and 21 with systemic disease) were isolated from whole blood. Poly(A) RNA was labeled using a commercial RNA amplification and labeling system (NuGEN Ovation), and gene expression profiles were obtained using commercial expression microarrays (Affymetrix HG\u2010U133 Plus 2.0).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>A total of 9,501 differentially expressed probe sets were identified among the JIA subtypes and controls (by analysis of variance; false discovery rate 5%). Specifically, 193, 1,036, 873, and 7,595 probe sets were different in PBMCs from the controls compared with those from the ERA, persistent oligoarthritis, RF\u2010negative polyarthritis, and systemic JIA patients, respectively. In patients with persistent oligoarthritis, RF\u2010negative polyarthritis, and systemic JIA subtypes, up\u2010regulation of genes associated with interleukin\u201010 (IL\u201010) signaling was prominent. A hemoglobin cluster was identified that was underexpressed in ERA patients but overexpressed in systemic JIA patients. The influence of JAK\/STAT, ERK\/MAPK, IL\u20102, and B cell receptor signaling pathways was evident in patients with persistent oligoarthritis. In systemic JIA, up\u2010regulation of innate immune pathways, including IL\u20106, Toll\u2010like receptor\/IL\u20101 receptor, and peroxisome proliferator\u2013activated receptor signaling, were noted, along with down\u2010regulation of gene networks related to natural killer cells and T cells. Complement and coagulation pathways were up\u2010regulated in systemic JIA, with a subset of these genes being differentially expressed in other subtypes as well.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Expression analysis identified differentially expressed genes in PBMCs obtained early in the disease from patients with different subtypes of JIA and in healthy controls, providing evidence of immunobiologic differences between these forms of childhood arthritis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.24601","type":"journal-article","created":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T11:05:41Z","timestamp":1246273541000},"page":"2102-2112","source":"Crossref","is-referenced-by-count":147,"title":["Subtype\u2010specific peripheral blood gene expression profiles in recent\u2010onset juvenile idiopathic arthritis"],"prefix":"10.1002","volume":"60","author":[{"given":"Michael G.","family":"Barnes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei A.","family":"Grom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan D.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Griffin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul","family":"Pavlidis","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lukasz","family":"Itert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ndate","family":"Fall","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dawn 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