{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T17:22:10Z","timestamp":1791566530603,"version":"4.3.4"},"reference-count":32,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T00:00:00Z","timestamp":1246233600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2009,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To identify the autoantigen recognized by the autoantibody that is associated with clinically amyopathic dermatomyositis (C\u2010ADM) and rapidly progressive interstitial lung disease (ILD).<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>An anti\u2013CADM\u2010140 antibody\u2013positive prototype serum sample was used to screen a HeLa cell\u2013derived complementary DNA (cDNA) library. Selected cDNA clones were further evaluated by immunoprecipitation of their in vitro\u2013transcribed and in vitro\u2013translated products using anti\u2013CADM\u2010140 antibody\u2013positive and anti\u2010CADM\u2010140 antibody\u2013negative sera. The lysates of COS\u20107 cells transfected with the putative antigen were similarly tested. An enzyme\u2010linked immunosorbent assay (ELISA) to detect the anti\u2013CADM\u2010140 antibody was established using a recombinant CADM\u2010140 antigen, and its specificity and sensitivity for C\u2010ADM and rapidly progressive ILD were assessed in 294 patients with various connective tissue diseases.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>By cDNA library screening and immunoprecipitation of in vitro\u2013transcribed and in vitro\u2013translated products, we obtained a cDNA clone encoding melanoma differentiation\u2013associated gene 5 (MDA\u20105). The anti\u2013CADM\u2010140 antibodies in patients' sera specifically reacted with MDA\u20105 protein expressed in cells transfected with full\u2010length MDA\u20105 cDNA, confirming the identity of MDA\u20105 as the CADM\u2010140 autoantigen. The ELISA, using recombinant MDA\u20105 protein as the antigen, showed an analytical sensitivity of 85% and analytical specificity of 100%, in comparison with the \u201cgold standard\u201d immunoprecipitation assay, and was useful for identifying patients with C\u2010ADM and\/or rapidly progressive ILD.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Given that RNA helicase encoded by MDA\u20105 is a critical molecule involved in the innate immune defense against viruses, viral infection may play an important role in the pathogenesis of C\u2010ADM and rapidly progressive ILD. Moreover, our ELISA using recombinant MDA\u20105 protein makes detection of the anti\u2013CADM\u2010140 antibody routinely available.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.24621","type":"journal-article","created":{"date-parts":[[2009,6,29]],"date-time":"2009-06-29T11:05:41Z","timestamp":1246273541000},"page":"2193-2200","source":"Crossref","is-referenced-by-count":545,"title":["RNA helicase encoded by melanoma differentiation\u2013associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease"],"prefix":"10.1002","volume":"60","author":[{"given":"Shinji","family":"Sato","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kana","family":"Hoshino","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Satoh","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomonobu","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yutaka","family":"Kawakami","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Takashi","family":"Fujita","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masataka","family":"Kuwana","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2009,6,29]]},"reference":[{"key":"e_1_2_7_2_2","first-page":"742","volume-title":"Arthritis and allied conditions","author":"Pearson CM","year":"1997"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1067\/mjd.2002.120621"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-007-0562-9"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21023"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350415"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780330908"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280711"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22164"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kel161"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0889-857X(02)00032-7"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780251101"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780230510"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1164\/ajrccm.165.2.ats01"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1002\/ijc.11625"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10521"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0606699104"},{"key":"e_1_2_7_19_2","first-page":"1523","article-title":"Length\u2010dependent recognition of double\u2010stranded ribonucleic acids by retinoic acid\u2010inducible gene\u2010I and melanoma differentiation\u2010associated gene 5","volume":"7","author":"Kato H","year":"2008","journal-title":"J Exp Med"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0960-9822(02)00842-4"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1038\/nature04734"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.175.5.2851"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.biochi.2007.01.013"},{"issue":"9","key":"e_1_2_7_24_2","first-page":"S518","article-title":"Autoantibodies to transcriptional intermediary factor 1\u2010\u03b1 (TIF1\u2010\u03b1) in dermatomyositis","volume":"54","author":"Targoff IN","year":"2006","journal-title":"Arthritis Rheum"},{"issue":"9","key":"e_1_2_7_25_2","first-page":"S787","article-title":"Sera with autoantibodies to the MJ antigen react with NXP2","volume":"56","author":"Targoff IN","year":"2007","journal-title":"Arthritis Rheum"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22862"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1080\/08916930400022681"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2004.09.021"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601066103"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/304177a0"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780291109"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/0306-9877(88)90041-2"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.190.6.815"}],"container-title":["Arthritis &amp; 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