{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T15:27:14Z","timestamp":1791300434676,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,2,22]],"date-time":"2010-02-22T00:00:00Z","timestamp":1266796800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>A genetic polymorphism in the programmed death 1 (PD\u20101) gene encoding the coinhibitory PD\u20101 immunoreceptor, PD\u20101.3A, is associated with systemic lupus erythematosus (SLE). The aim of this study was to assess PD\u20101 receptor expression in patients with SLE, in comparison with relatives and unrelated healthy controls, and to identify correlations of lower expression levels of PD\u20101 receptor with the PD\u20101.3A genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Patients with SLE, patients' relatives, and unrelated healthy control subjects from Iceland and Sweden were studied. Peripheral blood mononuclear cells (PBMCs) were stimulated with anti\u2010CD3\/anti\u2010CD28, and PD\u20101 expression was analyzed by flow cytometry. PD\u20101.3A\/G genotyping was performed using polymerase chain reaction\u2013restriction fragment length polymorphism analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PD\u20101 expression on PBMCs was induced after antibody stimulation, showing increases of 2.1\u2010fold in SLE patients, 3.1\u2010fold in relatives, and 5.1\u2010fold in healthy controls. The frequency of PD\u20101+ cells was significantly lower in SLE patients compared with relatives and healthy controls. PD\u20101 expression on PD\u20101+ cells and on CD4+CD25+ T cells was significantly lower in SLE patients and relatives compared with healthy controls. PD\u20101 expression was significantly elevated on CD25<jats:sup>high<\/jats:sup> cells. Levels of PD\u20101 expression on CD25<jats:sup>high<\/jats:sup> and CD25<jats:sup>intermediate<\/jats:sup> cells were significantly lower in SLE patients compared with healthy controls. PD\u20101 was expressed on both FoxP3\u2212 and FoxP3+ cells. Lower expression of PD\u20101 was significantly correlated with the PD\u20101.3A\/G genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results demonstrate significantly lower PD\u20101 receptor expression in SLE patients and their relatives and reveal a significant correlation of lower PD\u20101 expression with the PD\u20101.3A allele. Thus, PD\u20101.3A may contribute to abnormalities in PD\u20101 receptor expression on CD4+CD25+ T cells in patients with SLE, providing support for an important role of the PD\u20101 pathway in SLE and, possibly, in other autoimmune diseases.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.27417","type":"journal-article","created":{"date-parts":[[2010,2,22]],"date-time":"2010-02-22T17:34:42Z","timestamp":1266860082000},"page":"1702-1711","source":"Crossref","is-referenced-by-count":60,"title":["Lower expression levels of the programmed death 1 receptor on CD4+CD25+ T cells and correlation with the PD\u20101.3A genotype in patients with systemic lupus 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