{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T21:34:06Z","timestamp":1789940046686,"version":"4.0.1"},"reference-count":45,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,3,10]],"date-time":"2010-03-10T00:00:00Z","timestamp":1268179200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2010,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Adipokines may influence inflammatory and\/or immune responses. This study was undertaken to examine whether adiponectin affects the production of prostaglandin E\n                      <jats:sub>2<\/jats:sub>\n                      (PGE\n                      <jats:sub>2<\/jats:sub>\n                      ) by rheumatoid arthritis synovial fibroblasts (RASFs).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Synovial tissue was obtained from patients with RA who were undergoing joint replacement surgery. Fibroblast\u2010like cells from the third or fourth passage were used as RASFs. Expression of adiponectin receptor messenger RNA (mRNA) and protein was detected. PGE\n                      <jats:sub>2<\/jats:sub>\n                      (converted from arachidonic acid) was measured by enzyme\u2010linked immunosorbent assay (ELISA). Expression of mRNA and protein for cyclooxygenase 2 (COX\u20102) and membrane\u2010associated PGE synthase 1 (mPGES\u20101), key enzymes involved in PGE\n                      <jats:sub>2<\/jats:sub>\n                      synthesis, was detected in RASFs. The effects of RNA interference (RNAi) targeting the adiponectin receptor genes and the receptor signal inhibitors were examined. The influence of adiponectin on NF\u2010\u03baB activation in RASFs was measured with an ELISA kit.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Adiponectin receptors were detected in RASFs. Adiponectin increased both COX\u20102 and mPGES\u20101 mRNA and protein expression by RASFs in a time\u2010 and concentration\u2010dependent manner. PGE\n                      <jats:sub>2<\/jats:sub>\n                      production by RASFs was also increased by the addition of adiponectin, and this increase was inhibited by RNAi for the adiponectin receptor gene, or coincubation with the receptor signal inhibitors. Enhancement of NF\u2010\u03baB activation by adiponectin as well as by interleukin\u20101\u03b2 was observed in RASFs.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>\n                      Our findings indicate that adiponectin induces COX\u20102 and mPGES\u20101 expression, resulting in the enhancement of PGE\n                      <jats:sub>2<\/jats:sub>\n                      production by RASFs. Thus, adiponectin may play a role in the pathogenesis of synovitis in RA patients.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.27450","type":"journal-article","created":{"date-parts":[[2010,3,10]],"date-time":"2010-03-10T14:57:26Z","timestamp":1268233046000},"page":"1641-1649","source":"Crossref","is-referenced-by-count":61,"title":["Adiponectin stimulates prostaglandin E\n                    <sub>2<\/sub>\n                    production in rheumatoid arthritis synovial fibroblasts"],"prefix":"10.1002","volume":"62","author":[{"given":"Natsuko","family":"Kusunoki","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kanako","family":"Kitahara","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fumiaki","family":"Kojima","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nahoko","family":"Tanaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kaichi","family":"Kaneko","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hirahito","family":"Endo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Toru","family":"Suguro","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shinichi","family":"Kawai","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,5,28]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1043-2760(00)00301-5"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1373\/clinchem.2004.032482"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1937"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1463-1326.2006.00610.x"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI36389"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1001\/jama.290.13.1709-c"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2006.09.002"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbadis.2006.06.008"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2005.046540"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.176.7.4468"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2008.01.017"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24789"},{"key":"e_1_2_7_14_2","first-page":"1836","article-title":"Coexpression 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