{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,20]],"date-time":"2026-10-20T14:43:32Z","timestamp":1792507412466,"version":"4.5.0"},"reference-count":22,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T00:00:00Z","timestamp":1269907200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2010,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To examine both the source of follistatin\u2010like protein 1 (FSTL\u20101) and the factors that induce its expression in arthritis, and to determine whether juvenile rheumatoid arthritis (JRA) is characterized by overexpression of FSTL\u20101.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>FSTL\u20101 expression patterns were analyzed by immunohistochemical staining of joint tissue derived from mice with collagen\u2010induced arthritis. Induction of FSTL\u20101 secretion was assessed in osteoblasts, adipocytes, and human fibroblast\u2010like synoviocytes in response to transforming growth factor \u03b2 (TGF\u03b2), interleukin\u20101\u03b2 (IL\u20101\u03b2), tumor necrosis factor \u03b1 (TNF\u03b1), and IL\u20106. In addition, sera and synovial fluid from children with oligoarticular, polyarticular, or systemic\u2010onset JRA were assayed for FSTL\u20101 using a custom enzyme\u2010linked immunosorbent assay. FSTL\u20101 concentrations in these patients were assessed for correlations with the erythrocyte sedimentation rate (ESR) and platelet count.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Immunohistochemical staining of murine joint sections demonstrated expression of FSTL\u20101 in all cell types of the mesenchymal lineage, including osteocytes, chondrocytes, adipocytes, and fibroblasts. FSTL\u20101 could be induced in osteoblasts, adipocytes, and human fibroblast\u2010like synoviocytes by TGF\u03b2, IL\u20101\u03b2, TNF\u03b1, and IL\u20106. The IL\u20101\u03b2 response was significantly greater than the TNF\u03b1 response (<jats:italic>P<\/jats:italic> &lt; 0.05). In human serum and synovial fluid, only those samples from children with the systemic\u2010onset JRA subtype had elevated concentrations of FSTL\u20101. The synovial fluid concentrations of FSTL\u20101 were 2\u20133\u2010fold higher than the serum concentrations. The elevation in serum FSTL\u20101 concentrations seen in children with systemic\u2010onset JRA correlated closely with elevations in the ESR and platelet count.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings demonstrate that the arthritic joint matrix is a major source of FSTL\u20101 and that IL\u20101\u03b2 is a central mediator of FSTL\u20101 secretion. Furthermore, FSTL\u20101 may represent a useful biomarker of disease activity in systemic\u2010onset JRA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.27485","type":"journal-article","created":{"date-parts":[[2010,3,30]],"date-time":"2010-03-30T16:05:45Z","timestamp":1269965145000},"page":"2510-2516","source":"Crossref","is-referenced-by-count":74,"title":["Follistatin\u2010like protein 1 is a mesenchyme\u2010derived inflammatory protein and may represent a biomarker for systemic\u2010onset juvenile rheumatoid arthritis"],"prefix":"10.1002","volume":"62","author":[{"given":"David C.","family":"Wilson","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anthony D.","family":"Marinov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Harry C.","family":"Blair","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel S.","family":"Bushnell","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan D.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yury","family":"Chaly","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Raphael","family":"Hirsch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,8,3]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780290216"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200003163421103"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10710"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2003.014886"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.10885"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.3181\/00379727-68-16375"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI117969"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.2.6182.97"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1993.tb18212.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1006\/clim.2002.5227"},{"key":"e_1_2_6_12_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.182.1.234"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/10.9.1305"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20023"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/dxg005"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.7.4758"},{"key":"e_1_2_6_17_2","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.4049\/jimmunol.153.7.3319","article-title":"Induction of T helper cell hyporesponsiveness in an experimental model of autoimmunity using nonmitogenic anti\u2010CD3 monoclonal antibody","volume":"153","author":"Hughes C","year":"1994","journal-title":"J Immunol"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20050473"},{"key":"e_1_2_6_19_2","first-page":"S437","article-title":"Treatment of systemic onset juvenile rheumatoid arthritis with anakinra","volume":"50","author":"Irigoyen PI","year":"2004","journal-title":"Arthritis Rheum"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20357"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200306193482523"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(04)17401-1"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20953"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.27485","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.27485","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T20:52:36Z","timestamp":1698785556000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.27485"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,8]]},"references-count":22,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2010,8]]}},"alternative-id":["10.1002\/art.27485"],"URL":"https:\/\/doi.org\/10.1002\/art.27485","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,8]]}}}