{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T11:52:51Z","timestamp":1791114771184,"version":"4.1.0"},"reference-count":15,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2010,6,25]],"date-time":"2010-06-25T00:00:00Z","timestamp":1277424000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2010,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To substantiate a pathogenic role of cytotoxic CD8 T cells in the development of a murine polymyositis model, C protein\u2013induced myositis (CIM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Beta<jats:sub>2<\/jats:sub>\u2010microglobulin\u2013null mutant, perforin\u2010null mutant, and wild\u2010type (WT) C57BL\/6 mice were immunized with skeletal muscle C protein fragments to provoke CIM. Regional lymph node CD8 or CD4 T cells stimulated with C protein\u2013pulsed dendritic cells were transferred adoptively to naive mice. Inflammation and damage of the muscle tissues were evaluated histologically.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The incidence of myositis development was significantly lower in \u03b2<jats:sub>2<\/jats:sub>\u2010microglobulin\u2013null and perforin\u2010null mutant mice compared with WT mice. Inflammation was less severe in mutant mice, and the incidence of muscle injury was reduced significantly. Adoptive transfer of lymph node T cells from mice with CIM induced myositis in naive recipient mice. The CD8 T cell\u2013induced muscle injuries were significantly more severe than the CD4 T cell\u2013induced muscle injuries.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Perforin\u2010mediated cytotoxicity by CD8 T cells is definitively responsible for muscle injury in CIM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.27625","type":"journal-article","created":{"date-parts":[[2010,6,25]],"date-time":"2010-06-25T15:21:28Z","timestamp":1277479288000},"page":"3088-3092","source":"Crossref","is-referenced-by-count":41,"title":["Definitive engagement of cytotoxic CD8 T cells in C protein\u2013induced myositis, a murine model of polymyositis"],"prefix":"10.1002","volume":"62","author":[{"given":"Takahiko","family":"Sugihara","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naoko","family":"Okiyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mihoko","family":"Suzuki","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kuniko","family":"Kohyama","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yoh","family":"Matsumoto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nobuyuki","family":"Miyasaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hitoshi","family":"Kohsaka","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,6,25]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1759-1961.2009.00002.x"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22521"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1007\/BF00227760"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0601956103"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22846"},{"key":"e_1_2_7_7_2","first-page":"117","article-title":"Experimental autoimmune myositis in SJL\/J mice","volume":"68","author":"Rosenberg NL","year":"1987","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.7.989"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.79.23.14640-14646.2005"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200002)43:2<336::AID-ANR13>3.0.CO;2-V"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.183.1.67"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.7761837"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.21103"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1097-4598(199706)20:6<702::AID-MUS7>3.0.CO;2-C"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.10.5459"},{"key":"e_1_2_7_16_2","first-page":"S923","article-title":"A randomized, double\u2010blind, placebo\u2010controlled trial of infliximab in patients with polymyositis and dermatomyositis","volume":"58","author":"Coyle K","year":"2008","journal-title":"Arthritis Rheum"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.27625","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.27625","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T01:16:37Z","timestamp":1698714997000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.27625"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,6,25]]},"references-count":15,"journal-issue":{"issue":"10","published-print":{"date-parts":[[2010,10]]}},"alternative-id":["10.1002\/art.27625"],"URL":"https:\/\/doi.org\/10.1002\/art.27625","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,6,25]]}}}