{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,2]],"date-time":"2026-07-02T00:20:19Z","timestamp":1782951619986,"version":"3.54.5"},"reference-count":25,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2010,7,26]],"date-time":"2010-07-26T00:00:00Z","timestamp":1280102400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2010,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To explore biologic correlates to age at onset in patients with juvenile idiopathic arthritis (JIA) using peripheral blood mononuclear cell (PBMC) gene expression analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>PBMCs were isolated from 56 healthy controls and 104 patients with recent\u2010onset JIA (39 with persistent oligoarticular JIA, 45 with rheumatoid factor\u2013negative polyarticular JIA, and 20 with systemic JIA). RNA was amplified and labeled using NuGEN Ovation, and gene expression was assessed with Affymetrix HG\u2010U133 Plus 2.0 GeneChips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 832 probe sets revealed gene expression differences (false discovery rate 5%) in PBMCs from children with oligoarticular JIA whose disease began before age 6 years (early\u2010onset disease) compared with those whose disease began at or after age 6 years (late\u2010onset disease). In patients with early\u2010onset disease, there was greater expression of genes related to B cells and less expression of genes related to cells of the myeloid lineage. Support vector machine analyses identified samples from patients with early\u2010 or late\u2010onset oligoarticular JIA (with 97% accuracy) or from patients with early\u2010 or late\u2010onset polyarticular JIA (with 89% accuracy), but not from patients with systemic JIA or healthy controls. Principal components analysis showed that age at onset was the major classifier of samples from patients with oligoarticular JIA and patients with polyarticular JIA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>PBMC gene expression analysis reveals biologic differences between patients with early\u2010and late\u2010onset JIA, independent of classification based on the number of joints involved. These data suggest that age at onset may be an important parameter to consider in JIA classification. Furthermore, pathologic mechanisms may vary with age at onset, and understanding these processes may lead to improved treatment of JIA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.27657","type":"journal-article","created":{"date-parts":[[2010,7,26]],"date-time":"2010-07-26T17:49:23Z","timestamp":1280166563000},"page":"3249-3258","source":"Crossref","is-referenced-by-count":73,"title":["Biologic similarities based on age at onset in oligoarticular and polyarticular subtypes of juvenile idiopathic arthritis"],"prefix":"10.1002","volume":"62","author":[{"given":"Michael G.","family":"Barnes","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexei A.","family":"Grom","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Susan D.","family":"Thompson","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas A.","family":"Griffin","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lorie K.","family":"Luyrink","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Robert A.","family":"Colbert","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David N.","family":"Glass","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2010,10,29]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.27561"},{"key":"e_1_2_7_3_2","first-page":"1991","article-title":"Revision of the proposed classification criteria for juvenile idiopathic arthritis: Durban, 1997","volume":"25","author":"Petty RE","year":"1998","journal-title":"J Rheumatol"},{"key":"e_1_2_7_4_2","first-page":"390","article-title":"International League of Associations for Rheumatology classification of juvenile idiopathic arthritis: second revision, Edmonton, 2001","volume":"31","author":"Petty RE","year":"2004","journal-title":"J Rheumatol"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20815"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24601"},{"key":"e_1_2_7_7_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22981"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24534"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh224"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.45.6.464"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI109872"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(199909)42:9<1843::AID-ANR8>3.0.CO;2-M"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.27424"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.molimm.2008.05.011"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.20945"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gng015"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1198\/016214507000001120"},{"key":"e_1_2_7_18_2","first-page":"239","article-title":"Different circulating lymphocyte profiles in patients with different subtypes of juvenile idiopathic arthritis","volume":"20","author":"Wouters CH","year":"2002","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.05.012"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2164-7-115"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.179.6.3662"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar2824"},{"key":"e_1_2_7_23_2","first-page":"1900","article-title":"Are the number of joints involved or the presence of psoriasis still useful tools to identify homogeneous disease entities in juvenile idiopathic arthritis?","volume":"30","author":"Martini A","year":"2003","journal-title":"J Rheumatol"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kel225"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaci.2003.07.003"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0801888"}],"container-title":["Arthritis &amp; 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