{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T11:17:02Z","timestamp":1779189422021,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2011,10,28]],"date-time":"2011-10-28T00:00:00Z","timestamp":1319760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2011,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To investigate P\u2010 and E\u2010selectin ligand coexpression with chemokine receptors (CKRs) on T cells in the synovial fluid (SF) and blood of children with juvenile idiopathic arthritis (JIA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixteen patients with polyarticular or persistent oligoarticular JIA (ages 5.3\u201315.1 years) were studied. SF and venous blood were collected, and immunostaining for the expression of CCR4, CCR5, CXCR3, and P\u2010 or E\u2010selectin ligands was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Compared to blood, SF was greatly enriched for CD4+ T cells bearing CCR5, CCR4, CXCR3, and both P\u2010 and E\u2010selectin ligand. Twenty\u2010five percent of the CD4+ T cells in SF expressed both CCR5 and CCR4, some also coexpressing CXCR3. Such cells were rare in blood. Half of the few CCR5+ T cells in blood coexpressed P\u2010 or E\u2010selectin ligand, a phenotype that was enriched up to 50\u2010fold in SF. A minority of CCR4+ and CXCR3+ cells in blood (\u223c25%) coexpressed selectin ligand; these were enriched 4\u20138\u2010fold in SF. Most CCR4\u2010expressing CD4+ T cells expressed both E\u2010selectin ligand and cutaneous lymphocyte antigen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>CCR4\u2010, CCR5\u2010, CXCR3\u2010, and selectin ligand\u2013expressing CD4+ T cells preferentially accumulate in the joints of children with JIA. The marked enrichment of CCR5+ T cells coexpressing P\u2010selectin and\/or E\u2010selectin ligand in CD4+ SF T cells suggests that the few such cells in blood selectively migrate to inflamed joints via endothelial P\u2010 and E\u2010selectin\u2013 and CCR5\u2010activating chemokines. The predominance of CCR4\u2010expressing CD4+ T cells coexpressing E\u2010selectin ligand suggests that such cells migrate not only to areas of cutaneous inflammation, as previously reported, but also to the joints in JIA. Combined targeting of CCR5\u2010 and E\u2010selectin\u2013dependent mechanisms may be a relevant treatment strategy.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.30521","type":"journal-article","created":{"date-parts":[[2011,7,7]],"date-time":"2011-07-07T18:06:13Z","timestamp":1310061973000},"page":"3467-3476","source":"Crossref","is-referenced-by-count":15,"title":["Coexpression of chemokine receptors CCR5, CXCR3, and CCR4 and ligands for P\u2010 and E\u2010selectin on T lymphocytes of patients with juvenile idiopathic arthritis"],"prefix":"10.1002","volume":"63","author":[{"given":"Andrew C.","family":"Issekutz","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Patrick J.","family":"Quinn","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca","family":"Lang","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Suzanne","family":"Ramsey","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adam M.","family":"Huber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Derek","family":"Rowter","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohan","family":"Karkada","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thomas B.","family":"Issekutz","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2011,10,28]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.pharmtox.48.121806.154841"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.2741\/3261"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri2094"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1044-5323(02)00124-0"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.pathophys.2005.11.001"},{"key":"e_1_2_7_7_2","first-page":"5","article-title":"The pathogenesis of rheumatoid arthritis","volume":"36","author":"Koch AE","year":"2007","journal-title":"Am J Orthop (Belle Mead NJ)"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.081074"},{"key":"e_1_2_7_9_2","first-page":"451","article-title":"T cell chemokine receptor expression in human Th1\u2010 and Th2\u2010associated diseases","volume":"48","author":"Campbell JD","year":"2000","journal-title":"Arch Immunol Ther Exp (Warsz)"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200004)43:4<765::AID-ANR7>3.0.CO;2-B"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1385\/IR:31:1:57"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.2174\/138955707782331768"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI13543"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)64378-7"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1093\/intimm\/12.12.1659"},{"key":"e_1_2_7_16_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM200010053431407"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.166.11.6899"},{"key":"e_1_2_7_18_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.23291"},{"key":"e_1_2_7_19_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.1.122"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.2174\/187152809788681001"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1471-4914(03)00071-6"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/40.9.965"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.61.3.242"},{"key":"e_1_2_7_24_2","first-page":"249","article-title":"Soluble adhesion molecules ICAM\u20101 and E\u2010selectin in juvenile arthritis: clinical and laboratory correlations","volume":"20","author":"Dolezalova P","year":"2002","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_7_25_2","first-page":"366","article-title":"Soluble intercellular adhesion molecule\u20101 and E\u2010selectin as markers of disease activity and endothelial activation in juvenile idiopathic arthritis","volume":"32","author":"Bloom BJ","year":"2005","journal-title":"J Rheumatol"},{"key":"e_1_2_7_26_2","doi-asserted-by":"publisher","DOI":"10.1002\/(SICI)1521-4141(199904)29:04<1094::AID-IMMU1094>3.0.CO;2-6"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830270628"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2009.00795.x"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200106)44:6<1428::AID-ART238>3.0.CO;2-U"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1038\/385081a0"},{"key":"e_1_2_7_31_2","doi-asserted-by":"crossref","first-page":"3193","DOI":"10.4049\/jimmunol.162.6.3193","article-title":"Expression of functional selectin ligands on Th cells is differentially regulated by IL\u201012 and IL\u20104","volume":"162","author":"Lim YC","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.177.11.7673"},{"key":"e_1_2_7_33_2","first-page":"390","article-title":"International League of Associations for Rheumatology classification of juvenile idiopathic arthritis: second revision, Edmonton, 2001","volume":"31","author":"Petty RE","year":"2004","journal-title":"J Rheumatol"},{"key":"e_1_2_7_34_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.174.6.1461"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-065X.2002.18603.x"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2009.04.002"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1189\/jlb.1102547"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2006.061853"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1913"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar1481"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364298"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1038\/gene.2010.25"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1080\/03009740701631935"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364317"},{"key":"e_1_2_7_45_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00166-2"},{"key":"e_1_2_7_46_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-065X.2009.00797.x"},{"key":"e_1_2_7_47_2","doi-asserted-by":"publisher","DOI":"10.1002\/1521-4141(200211)32:11<3171::AID-IMMU3171>3.0.CO;2-4"},{"key":"e_1_2_7_48_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780390118"},{"key":"e_1_2_7_49_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/36.7.748"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.30521","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.30521","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,11]],"date-time":"2023-10-11T17:56:00Z","timestamp":1697046960000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.30521"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2011,10,28]]},"references-count":48,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2011,11]]}},"alternative-id":["10.1002\/art.30521"],"URL":"https:\/\/doi.org\/10.1002\/art.30521","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2011,10,28]]}}}