{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,12]],"date-time":"2026-04-12T08:13:23Z","timestamp":1775981603308,"version":"3.50.1"},"reference-count":43,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,2,28]],"date-time":"2012-02-28T00:00:00Z","timestamp":1330387200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH","award":["AR-060169"],"award-info":[{"award-number":["AR-060169"]}]},{"name":"NIH","award":["AR-055600"],"award-info":[{"award-number":["AR-055600"]}]},{"name":"NIH","award":["AI-039824"],"award-info":[{"award-number":["AI-039824"]}]},{"name":"NIH","award":["AR-050812"],"award-info":[{"award-number":["AR-050812"]}]},{"name":"NIH","award":["AR-055240"],"award-info":[{"award-number":["AR-055240"]}]},{"name":"NIH","award":["AR-050250"],"award-info":[{"award-number":["AR-050250"]}]},{"name":"NIH","award":["AR-054796"],"award-info":[{"award-number":["AR-054796"]}]},{"name":"NIH","award":["AR-055503"],"award-info":[{"award-number":["AR-055503"]}]},{"name":"NIH","award":["AI-067590"],"award-info":[{"award-number":["AI-067590"]}]},{"DOI":"10.13039\/100008250","name":"Northwestern University Feinberg School of Medicine","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100008250","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2012,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>The death receptor Fas is a critical mediator of the extrinsic apoptotic pathway, and its role in mediating lymphoproliferation has been extensively examined. The present study was undertaken to investigate the impact of myeloid cell\u2013specific loss of Fas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice with Fas flanked by <jats:italic>loxP<\/jats:italic> sites (Fas<jats:sup>flox\/flox<\/jats:sup>) were crossed with mice expressing Cre under control of the murine lysozyme M gene promoter (Cre<jats:sup>LysM<\/jats:sup>), which functions in mature lysozyme\u2010expressing cells of the myelomonocytic lineage. The genotype for Cre<jats:sup>LysM<\/jats:sup>Fas<jats:sup>flox\/flox<\/jats:sup> mice was verified by polymerase chain reaction and flow cytometric analysis. Flow cytometric analysis was also used to characterize myeloid, dendritic, and lymphoid cell distribution and activation in bone marrow, blood, and spleen. Luminex\u2010based assays and enzyme\u2010linked immunosorbent assays were used to measure serum cytokine\/chemokine and immunoglobulin levels. Renal damage or dysfunction was examined by immunohistochemical and immunofluorescence analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Cre<jats:sup>LysM<\/jats:sup>Fas<jats:sup>flox\/flox<\/jats:sup> mice exhibited a systemic lupus erythematosus (SLE)\u2013like disease that included leukocytosis, splenomegaly, hypergammaglobulinemia, antinuclear autoantibody and proinflammatory cytokine production, and glomerulonephritis. Loss of Fas in myeloid cells increased levels of both Gr\u20101<jats:sup>low<\/jats:sup> and Gr\u20101<jats:sup>intermediate<\/jats:sup> blood monocytes and splenic macrophages and, in a paracrine manner, incited activation of conventional dendritic cells and lymphocytes in Cre<jats:sup>LysM<\/jats:sup>Fas<jats:sup>flox\/flox<\/jats:sup> mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Taken together, these results suggest that loss of Fas in myeloid cells is sufficient to induce inflammatory phenotypes in mice, reminiscent of an SLE\u2010like disease. Thus, Fas in myeloid cells may be considered a suppressor of systemic autoimmunity.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.34317","type":"journal-article","created":{"date-parts":[[2011,12,5]],"date-time":"2011-12-05T18:36:28Z","timestamp":1323110188000},"page":"808-820","source":"Crossref","is-referenced-by-count":24,"title":["Requirement of myeloid cell\u2013specific Fas expression for prevention of systemic autoimmunity in mice"],"prefix":"10.1002","volume":"64","author":[{"given":"Carla M.","family":"Cuda","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hemant","family":"Agrawal","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alexander V.","family":"Misharin","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"suffix":"III","given":"G. Kenneth","family":"Haines","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jack","family":"Hutcheson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Evan","family":"Weber","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Joseph A.","family":"Schoenfeldt","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chandra","family":"Mohan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Richard M.","family":"Pope","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Harris","family":"Perlman","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2012,2,28]]},"reference":[{"key":"e_1_2_6_2_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.01.001"},{"key":"e_1_2_6_3_2","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.iy.09.040191.001331"},{"key":"e_1_2_6_4_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.03.016"},{"key":"e_1_2_6_5_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20032196"},{"key":"e_1_2_6_6_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.07.016"},{"key":"e_1_2_6_7_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2007.12.015"},{"key":"e_1_2_6_8_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20030698"},{"key":"e_1_2_6_9_2","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20041484"},{"key":"e_1_2_6_10_2","doi-asserted-by":"publisher","DOI":"10.1046\/j.1523-1755.2000.00272.x"},{"key":"e_1_2_6_11_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1117729"},{"key":"e_1_2_6_12_2","doi-asserted-by":"crossref","first-page":"5303","DOI":"10.4049\/jimmunol.163.10.5303","article-title":"Dendritic cells are resistant to apoptosis through the Fas (CD95\/APO\u20101) pathway","volume":"163","author":"Ashany D","year":"1999","journal-title":"J Immunol"},{"key":"e_1_2_6_13_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00299-1"},{"key":"e_1_2_6_14_2","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V96.2.719"},{"key":"e_1_2_6_15_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2009.03944.x"},{"key":"e_1_2_6_16_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.clim.2008.01.019"},{"key":"e_1_2_6_17_2","doi-asserted-by":"publisher","DOI":"10.3109\/08916930108994122"},{"key":"e_1_2_6_18_2","doi-asserted-by":"publisher","DOI":"10.1186\/ar2964"},{"key":"e_1_2_6_19_2","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2005.038315"},{"key":"e_1_2_6_20_2","doi-asserted-by":"publisher","DOI":"10.1177\/0961203310392432"},{"key":"e_1_2_6_21_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0030491"},{"key":"e_1_2_6_22_2","doi-asserted-by":"publisher","DOI":"10.1016\/0161-5890(95)00024-9"},{"key":"e_1_2_6_23_2","doi-asserted-by":"publisher","DOI":"10.1002\/stem.68"},{"key":"e_1_2_6_24_2","first-page":"109","article-title":"IL\u20101\u03b2\u2010deficient mice are resistant to induction of experimental SLE","volume":"17","author":"Voronov E","year":"2006","journal-title":"Eur Cytokine Netw"},{"key":"e_1_2_6_25_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI1112"},{"key":"e_1_2_6_26_2","doi-asserted-by":"publisher","DOI":"10.1038\/sj.cdd.4400460"},{"key":"e_1_2_6_27_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.05.010"},{"key":"e_1_2_6_28_2","doi-asserted-by":"publisher","DOI":"10.1038\/ni.1863"},{"key":"e_1_2_6_29_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jbspin.2010.09.005"},{"key":"e_1_2_6_30_2","doi-asserted-by":"publisher","DOI":"10.1155\/2010\/498980"},{"key":"e_1_2_6_31_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2010.11.025"},{"key":"e_1_2_6_32_2","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(03)00240-1"},{"key":"e_1_2_6_33_2","doi-asserted-by":"publisher","DOI":"10.1126\/science.1085536"},{"key":"e_1_2_6_34_2","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M606721200"},{"key":"e_1_2_6_35_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.182.1.207"},{"key":"e_1_2_6_36_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0002-9440(10)63400-1"},{"key":"e_1_2_6_37_2","doi-asserted-by":"publisher","DOI":"10.1007\/s00281-006-0034-3"},{"key":"e_1_2_6_38_2","doi-asserted-by":"publisher","DOI":"10.1038\/nri1957"},{"key":"e_1_2_6_39_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.semarthrit.2008.11.002"},{"key":"e_1_2_6_40_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001180"},{"key":"e_1_2_6_41_2","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3001201"},{"key":"e_1_2_6_42_2","doi-asserted-by":"publisher","DOI":"10.1177\/0961203310371161"},{"key":"e_1_2_6_43_2","doi-asserted-by":"publisher","DOI":"10.1002\/humu.21565"},{"key":"e_1_2_6_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2010.02.007"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.34317","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.34317","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.34317","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,10]],"date-time":"2023-10-10T23:19:44Z","timestamp":1696979984000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.34317"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,2,28]]},"references-count":43,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2012,3]]}},"alternative-id":["10.1002\/art.34317"],"URL":"https:\/\/doi.org\/10.1002\/art.34317","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,2,28]]}}}