{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T11:07:10Z","timestamp":1790161630559,"version":"4.1.0"},"reference-count":55,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2012,7,27]],"date-time":"2012-07-27T00:00:00Z","timestamp":1343347200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2012,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Controversy surrounds the identity and functionality of rare bone marrow\u2013derived multipotential stromal cells (BM\u2010MSCs), including their differentiation capabilities, their relationship to pericytes and hematopoiesis\u2010supporting stromal cells, and the relevance of their culture\u2010expanded progeny in studies of skeletal biology and development of cell\u2010based therapies. The aim of this study was to clarify the nature of candidate BM\u2010MSCs by profiling transcripts that reflect different aspects of their putative functions in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Rare, sorted BM\u2010derived CD45<jats:sup>\u2212\/low<\/jats:sup> CD271<jats:sup>bright<\/jats:sup> (CD271) cells were analyzed using 96\u2010gene expression arrays focused on transcripts relevant to mesenchymal\u2010lineage differentiation (toward bone, cartilage, fat, or muscle), hematopoietic and stromal support, and molecules critical to skeletal homeostasis. These cells were compared to matched CD45+ CD271\u2212 hematopoietic\u2010lineage cells, culture\u2010expanded MSCs, and skin fibroblasts. When feasible, transcription was validated using flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>CD271 cells had a transcriptional profile consistent with the multiple fates of in vivo MSCs, evident from the observed simultaneous expression of osteogenic, adipogenic, pericytic, and hematopoiesis\u2010supporting genes (e.g., <jats:italic>SP7<\/jats:italic> [osterix], <jats:italic>FABP4<\/jats:italic> [fatty acid binding protein 4], <jats:italic>ANGPT1<\/jats:italic> [angiopoietin 1], and <jats:italic>CXCL12<\/jats:italic> [stromal cell\u2013derived factor 1], respectively). Compared to culture\u2010expanded MSCs and fibroblasts, CD271 cells exhibited greater transcriptional activity, particularly with respect to Wnt\u2010related genes (&gt;1,000\u2010fold increased expression of <jats:italic>FRZB<\/jats:italic> [secreted frizzled\u2010related protein 3] and <jats:italic>WIF1<\/jats:italic> [Wnt inhibitory factor 1]). A number of transcripts were identified as novel markers of MSCs.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The native, BM\u2010derived in vivo MSC population is endowed with a gene signature that is compatible with multiple functions, reflecting the topographic bone niche of these cells, and their signature is significantly different from that of culture\u2010expanded MSCs. This indicates that studies of the biologic functions of MSCs in musculoskeletal diseases, including osteoporosis and osteoarthritis, should focus on in vivo MSCs, rather than their culture\u2010adapted progeny.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.34434","type":"journal-article","created":{"date-parts":[[2012,2,29]],"date-time":"2012-02-29T17:51:16Z","timestamp":1330537876000},"page":"2632-2643","source":"Crossref","is-referenced-by-count":119,"title":["Transcriptional profile of native CD271+ multipotential stromal cells: Evidence for multiple fates, with prominent osteogenic and Wnt pathway signaling activity"],"prefix":"10.1002","volume":"64","author":[{"given":"Sarah M.","family":"Churchman","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Frederique","family":"Ponchel","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sally 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