{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,4]],"date-time":"2026-10-04T12:00:15Z","timestamp":1791115215589,"version":"4.1.0"},"reference-count":43,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2012,10,27]],"date-time":"2012-10-27T00:00:00Z","timestamp":1351296000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2012,11]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate the effect of duration of untreated disease on vascular cell adhesion molecule 1 (VCAM\u20101) and microRNA (miRNA) expression in muscle biopsy samples from children with juvenile dermatomyositis (DM) as well as its effect on soluble VCAM\u20101 (sVCAM\u20101) and tumor necrosis factor \u03b1 (TNF\u03b1) concentrations in sera from these children.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled 28 untreated children with juvenile DM and 8 pediatric controls. Eleven children with juvenile DM had short duration of untreated disease (symptoms for \u22642 months before muscle biopsy), and 17 had long duration of untreated disease (symptoms for &gt;2 months before muscle biopsy). Vascular structures, characterized by immunofluorescence using antibodies against von Willebrand factor, VCAM\u20101, and \u03b1\u2010smooth muscle actin, were measured for total area and intensity. Circulating sVCAM\u20101 and TNF\u03b1 levels were determined in patients with short duration of untreated disease, patients with long duration of untreated disease, and controls. Differential expression of microRNA\u2010126 (miR\u2010126) in muscle biopsy samples from the 2 patient groups and the control group was detected by miRNA expression profiling and confirmed by quantitative reverse transcription\u2013polymerase chain reaction in muscle biopsy samples from the 3 groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Juvenile DM patients with short duration of untreated disease had significantly higher total positive area and intensity\/high power field of VCAM\u20101 expression than did juvenile DM patients with long duration of untreated disease (<jats:italic>P<\/jats:italic> = 0.043 and <jats:italic>P<\/jats:italic> = 0.015, respectively) or controls (<jats:italic>P<\/jats:italic> = 0.004 and <jats:italic>P<\/jats:italic> = 0.001, respectively). Von Willebrand factor antigen\u2013positive vasculature displayed greater VCAM\u20101 intensity in patients with short duration of untreated disease than in patients with long duration of untreated disease (<jats:italic>P<\/jats:italic> = 0.001). Circulating levels of sVCAM\u20101 and TNF\u03b1 were significantly higher in patients with short duration of untreated disease than in controls (<jats:italic>P<\/jats:italic> = 0.013 and <jats:italic>P<\/jats:italic> = 0.048, respectively). The miRNA miR\u2010126, a negative regulator of VCAM\u20101 expression, was significantly decreased (3.39\u2010fold; <jats:italic>P<\/jats:italic> &lt; 0.006) in patients with short duration of untreated disease compared to controls, while miR\u2010126 expression in patients with long duration of untreated disease did not differ significantly compared to controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In patients with short duration of untreated disease, miR\u2010126 down\u2010regulation is associated with increased VCAM\u20101 in both muscle and blood, suggesting that VCAM\u20101 plays a critical role early in juvenile DM disease pathophysiology, augmented by TNF\u03b1.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.34606","type":"journal-article","created":{"date-parts":[[2012,6,27]],"date-time":"2012-06-27T19:48:38Z","timestamp":1340826518000},"page":"3809-3817","source":"Crossref","is-referenced-by-count":44,"title":["Increased expression of vascular cell adhesion molecule 1 in muscle biopsy samples from juvenile dermatomyositis patients with short duration of untreated disease is regulated by miR\u2010126"],"prefix":"10.1002","volume":"64","author":[{"given":"Erin","family":"Kim","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joan","family":"Cook\u2010Mills","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle","family":"Morgan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Simone T.","family":"Sredni","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren M.","family":"Pachman","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,10,27]]},"reference":[{"key":"e_1_2_7_2_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.11122"},{"key":"e_1_2_7_3_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"e_1_2_7_4_2","doi-asserted-by":"publisher","DOI":"10.1097\/00005792-196607000-00001"},{"key":"e_1_2_7_5_2","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.26.10.952"},{"key":"e_1_2_7_6_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.23299"},{"key":"e_1_2_7_7_2","first-page":"1644","article-title":"Skin involvement in juvenile dermatomyositis is associated with loss of end row nailfold capillary loops","volume":"31","author":"Smith RL","year":"2004","journal-title":"J Rheumatol"},{"key":"e_1_2_7_8_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.23341"},{"key":"e_1_2_7_9_2","doi-asserted-by":"publisher","DOI":"10.1186\/1740-2557-3-2"},{"key":"e_1_2_7_10_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2005.10.032"},{"key":"e_1_2_7_11_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.22433"},{"key":"e_1_2_7_12_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.24936"},{"key":"e_1_2_7_13_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.8.4154"},{"key":"e_1_2_7_14_2","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2172-9-43"},{"key":"e_1_2_7_15_2","doi-asserted-by":"publisher","DOI":"10.1016\/0022-510X(89)90228-1"},{"key":"e_1_2_7_16_2","first-page":"467","article-title":"Leucocyte\/endothelial cell adhesion receptors in muscle biopsies from patients with idiopathic inflammatory myopathies (IIM)","volume":"104","author":"Cid MC","year":"1996","journal-title":"Clin Exp Immunol"},{"key":"e_1_2_7_17_2","doi-asserted-by":"publisher","DOI":"10.1002\/mus.10462"},{"key":"e_1_2_7_18_2","first-page":"33","article-title":"Increased expression of cell adhesion molecules in inflammatory myopathies: diagnostic utility and pathogenetic insights","volume":"47","author":"Jain A","year":"2009","journal-title":"Folia Neuropathol"},{"key":"e_1_2_7_19_2","first-page":"801","article-title":"Immunohistochemical analysis of adhesion molecule expression on muscle biopsy specimens from patients with juvenile dermatomyositis","volume":"31","author":"Sallum AM","year":"2004","journal-title":"J Rheumatol"},{"key":"e_1_2_7_20_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.autrev.2005.05.008"},{"key":"e_1_2_7_21_2","doi-asserted-by":"publisher","DOI":"10.1016\/0165-5728(95)00045-4"},{"key":"e_1_2_7_22_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.9.3943"},{"key":"e_1_2_7_23_2","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(92)90633-N"},{"key":"e_1_2_7_24_2","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMe020157"},{"key":"e_1_2_7_25_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0049-0172(96)80034-5"},{"key":"e_1_2_7_26_2","first-page":"87","volume-title":"Adhesion molecules in health and disease","author":"Lebranchu Y","year":"1997"},{"key":"e_1_2_7_27_2","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200010)43:10<2368::AID-ANR26>3.0.CO;2-8"},{"key":"e_1_2_7_28_2","doi-asserted-by":"publisher","DOI":"10.1172\/JCI11871"},{"key":"e_1_2_7_29_2","doi-asserted-by":"publisher","DOI":"10.1186\/1756-8722-3-37"},{"key":"e_1_2_7_30_2","doi-asserted-by":"publisher","DOI":"10.1101\/gad.1184404"},{"key":"e_1_2_7_31_2","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/36.2.164"},{"key":"e_1_2_7_32_2","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1994.tb06107.x"},{"key":"e_1_2_7_33_2","doi-asserted-by":"publisher","DOI":"10.1016\/S0190-9622(00)90010-0"},{"key":"e_1_2_7_34_2","first-page":"832","article-title":"Soluble adhesion molecules in pediatric rheumatic diseases","volume":"29","author":"Bloom BJ","year":"2002","journal-title":"J Rheumatol"},{"key":"e_1_2_7_35_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780350606"},{"key":"e_1_2_7_36_2","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1001782"},{"key":"e_1_2_7_37_2","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780371206"},{"key":"e_1_2_7_38_2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0707493105"},{"key":"e_1_2_7_39_2","doi-asserted-by":"publisher","DOI":"10.1097\/MOT.0b013e3283446c52"},{"key":"e_1_2_7_40_2","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvn156"},{"key":"e_1_2_7_41_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.yexmp.2011.04.016"},{"key":"e_1_2_7_42_2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0017343"},{"key":"e_1_2_7_43_2","doi-asserted-by":"publisher","DOI":"10.1097\/MD.0b013e31816bc604"},{"key":"e_1_2_7_44_2","doi-asserted-by":"publisher","DOI":"10.1016\/j.jpeds.2011.05.015"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.34606","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.34606","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,31]],"date-time":"2023-10-31T02:33:12Z","timestamp":1698719592000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.34606"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,10,27]]},"references-count":43,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2012,11]]}},"alternative-id":["10.1002\/art.34606"],"URL":"https:\/\/doi.org\/10.1002\/art.34606","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,10,27]]}}}