{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T18:51:12Z","timestamp":1791399072972,"version":"4.3.3"},"reference-count":54,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T00:00:00Z","timestamp":1366675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Through its location on nociceptors, acid\u2010sensing ion channel 3 (ASIC\u20103) is activated by decreases in pH and plays a significant role in musculoskeletal pain. We recently showed that decreases in pH activate ASIC\u20103 located on fibroblast\u2010like synoviocytes (FLS), which are key cells in the inflammatory process. The purpose of this study was to test whether ASIC\u20103\u2013deficient mice with arthritis have altered inflammation and pain relative to controls.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Collagen antibody\u2013induced arthritis (CAIA) was generated by injection of an anti\u2013type II collagen antibody cocktail. Inflammation and pain parameters in ASIC\u20103<jats:sup>\u2212\/\u2212<\/jats:sup> and ASIC\u20103<jats:sup>+\/+<\/jats:sup> mice were assessed. Disease severity was assessed by determining clinical arthritis scores, measuring joint diameters, analyzing joint histology, and assessing synovial gene expression by quantitative polymerase chain reaction analysis. Cell death was assessed with a Live\/Dead assay of FLS in response to decreases in pH. Pain behaviors in the mice were measured by examining withdrawal thresholds in the joints and paws and by measuring their physical activity levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Surprisingly, ASIC\u20103<jats:sup>\u2212\/\u2212<\/jats:sup> mice with CAIA demonstrated significantly increased joint inflammation, joint destruction, and expression of interleukin\u20106 (IL\u20106), matrix metalloproteinase 3 (MMP\u20103), and MMP\u201013 in joint tissue as compared to ASIC\u20103<jats:sup>+\/+<\/jats:sup> mice. ASIC\u20103<jats:sup>+\/+<\/jats:sup> FLS showed enhanced cell death when exposed to pH 6.0 in the presence of IL\u20101\u03b2, which was abolished in ASIC\u20103<jats:sup>\u2212\/\u2212<\/jats:sup> FLS. Despite enhanced disease severity, ASIC\u20103<jats:sup>\u2212\/\u2212<\/jats:sup> mice did not develop mechanical hypersensitivity of the paw and showed greater levels of physical activity.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings are consistent with the hypothesis that ASIC\u20103 plays a protective role in the inflammatory arthritides by limiting inflammation through enhanced synoviocyte cell death, which reduces disease severity, and through the production of pain, which reduces joint use.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.37862","type":"journal-article","created":{"date-parts":[[2013,1,17]],"date-time":"2013-01-17T20:56:50Z","timestamp":1358456210000},"page":"1194-1202","source":"Crossref","is-referenced-by-count":61,"title":["Acid\u2010Sensing Ion Channel 3 Deficiency Increases Inflammation but Decreases Pain Behavior in Murine Arthritis"],"prefix":"10.1002","volume":"65","author":[{"given":"Kathleen 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