{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,27]],"date-time":"2026-10-27T07:52:56Z","timestamp":1793087576714,"version":"4.5.0"},"reference-count":44,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,4,23]],"date-time":"2013-04-23T00:00:00Z","timestamp":1366675200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2013,5]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Objective<\/jats:title><jats:p>Lyme arthritis (LA) is characterized by infiltration of inflammatory cells, mainly neutrophils (polymorphonuclear cells [PMNs]) and T cells, into the joints. This study was undertaken to evaluate the role of the neutrophil\u2010activating protein A (NapA) of <jats:italic>Borrelia burgdorferi<\/jats:italic> in eliciting inflammation and in driving the adaptive immune response.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Levels of NapA, interferon\u2010\u03b3 (IFN\u03b3), interleukin\u201017 (IL\u201017), and T cell\u2013attracting chemokines were assessed by enzyme\u2010linked immunosorbent assay in synovial fluid from patients with LA. The profile of T cells recruited into the synovia of patients with LA was defined by fluorescence\u2010activated cell sorting analysis. NapA was intraarticularly injected into rat knees, and the cells recruited in synovia were characterized. The role of NapA in recruiting immune cells was confirmed by chemotaxis assays using a Transwell system.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>NapA, IFN\u03b3, IL\u201017, CCL2, CCL20, and CXCL10 accumulated in synovial fluid from patients with LA. Accordingly, T cells obtained from these patients produced IFN\u03b3 or IL\u201017, but notably, some produced both cytokines. NapA promoted neutrophil and T lymphocyte recruitment both in vitro and in vivo. Interestingly, the infiltration of T cells not only resulted from the chemotactic activity of NapA but also relied on the chemokines produced by PMNs exposed to NapA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We provide evidence that NapA functions as one of the main bacterial products involved in the pathogenesis of LA. Accordingly, we show that, at very early stages of LA, NapA accumulates and, in turn, orchestrates the recruitment of inflammatory cells into the joint cavity. Thereafter, with the contribution of recruited cells, NapA promotes the infiltration of T cells producing IL\u201017 and\/or IFN\u03b3.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.37875","type":"journal-article","created":{"date-parts":[[2013,1,31]],"date-time":"2013-01-31T18:25:09Z","timestamp":1359656709000},"page":"1232-1242","source":"Crossref","is-referenced-by-count":37,"title":["Orchestration of Inflammation and Adaptive Immunity in <i>Borrelia burgdorferi<\/i>\u2013Induced Arthritis by Neutrophil\u2010Activating Protein A"],"prefix":"10.1002","volume":"65","author":[{"given":"Gaia","family":"Codolo","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fleur","family":"Bossi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paolo","family":"Durigutto","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiara 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