{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T02:32:27Z","timestamp":1791340347786,"version":"4.3.1"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,5,30]],"date-time":"2013-05-30T00:00:00Z","timestamp":1369872000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>The interleukin\u201012 (IL\u201012) family of cytokines has been suggested to play a critical role in inflammatory autoimmune diseases, and recent studies analyzing peripheral blood and synovial fluid from patients with spondyloarthritides suggest that IL\u201023 might be a proinflammatory factor in these disorders. This study was undertaken to investigate the presence and source of IL\u201023 in the spines of patients with ankylosing spondylitis (AS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The frequency of IL\u201023\u2013positive and IL\u201012\u2013positive cells within the subchondral bone marrow and within fibrous tissue replacing normal bone marrow in facet joints of patients with AS was analyzed by immunohistochemistry. The origin of IL\u201023\u2013positive cells was determined by double staining of CD163+ macrophages, CD68+ macrophages, CD1a+ dendritic cells, tryptase\u2010positive mast cells, myeloperoxidase\u2010positive cells, CD20+ B cells, and CD3+ T cells. Findings in 28 facet joints from 22 AS patients were compared with those in 20 facet joints from 13 patients with osteoarthritis (OA) and 10 normal control specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequency of IL\u201023\u2013positive cells in subchondral bone marrow from the joints of AS patients (mean \u00b1 SD 42.50 \u00b1 32.81\/high\u2010power field [hpf]) was significantly increased compared to that in subchondral bone marrow from OA patients (OA 15.63 \u00b1 29.90\/hpf) (<jats:italic>P<\/jats:italic> = 0.0017) or controls (19.36 \u00b1 16.8\/hpf) (<jats:italic>P<\/jats:italic> = 0.03). Myeloperoxidase\u2010positive cells and, to a lesser extent, macrophages and dendritic cells were found to be the origin of IL\u201023 in the bone marrow. In AS and OA patients, the frequency of IL\u201023\u2013positive cells was significantly higher than that of IL\u201012\u2013positive cells (<jats:italic>P<\/jats:italic> &lt; 0.001 in both patient groups). Within fibrous tissue from AS and OA facet joints, IL\u201023 was predominantly produced by CD163+ macrophages (mean \u00b1 SD 0.64 \u00b1 0.59\/hpf and 4.36 \u00b1 3.4\/hpf, respectively) and CD68+ macrophages (2.3 \u00b1 0.65\/hpf and 6.54 \u00b1 4.1\/hpf, respectively).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>IL\u201023 is expressed in the subchondral bone marrow and in fibrous tissue replacing bone marrow in facet joints of patients with AS. It might have a role in inflammatory processes and in chronic changes in AS joints, which makes it an interesting potential therapeutic target in this disease.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.37937","type":"journal-article","created":{"date-parts":[[2013,3,18]],"date-time":"2013-03-18T21:15:12Z","timestamp":1363641312000},"page":"1522-1529","source":"Crossref","is-referenced-by-count":128,"title":["In Situ Analysis of Interleukin\u201323\u2013 and Interleukin\u201012\u2013Positive Cells in the Spine of Patients With Ankylosing Spondylitis"],"prefix":"10.1002","volume":"65","author":[{"given":"Heiner","family":"Appel","sequence":"first","affiliation":[{"name":"Charit\u00e9 Berlin, Campus Benjamin Franklin Berlin Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rene","family":"Maier","sequence":"additional","affiliation":[{"name":"Charit\u00e9 Berlin, Campus Benjamin Franklin Berlin 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Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joachim","family":"Sieper","sequence":"additional","affiliation":[{"name":"Charit\u00e9 Berlin, Campus Benjamin Franklin Berlin 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