{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T03:50:25Z","timestamp":1788493825040,"version":"build-2803163510"},"reference-count":12,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,7,2]],"date-time":"2013-07-02T00:00:00Z","timestamp":1372723200000},"content-version":"vor","delay-in-days":1,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To investigate whether citrullinated proteins within the atherosclerotic plaque can be targeted by anti\u2013citrullinated protein antibodies (ACPAs), forming stimulatory immune complexes that propagate the progression of atherosclerosis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Protein lysates prepared from atherosclerotic segments of human aorta were assessed for the presence of citrulline\u2010modified proteins, and specifically citrullinated fibrinogen (Cit\u2010fibrinogen), by immunoprecipitation and\/or immunoblotting followed by mass spectrometry. Immunohistochemical analysis of coronary artery plaque was performed to determine the presence of citrullinated proteins and peptidylarginine deiminase type 4 (PAD\u20104). Serum levels of anti\u2013cyclic citrullinated peptide (anti\u2010CCP), anti\u2013citrullinated vimentin (anti\u2013Cit\u2010vimentin), and anti\u2013Cit\u2010fibrinogen antibodies were measured in 134 women with seropositive rheumatoid arthritis; these subjects had previously been characterized for the presence of subclinical atherosclerosis, by electron beam computed tomography scanning.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>Western blot analysis of atherosclerotic plaque lysates demonstrated several citrullinated proteins, and the presence of Cit\u2010fibrinogen was confirmed by immunoprecipitation and mass spectrometry. Immunohistochemical analysis showed colocalization of citrullinated proteins and PAD\u20104 within the coronary artery plaque. In age\u2010adjusted regression models, antibodies targeting Cit\u2010fibrinogen and Cit\u2010vimentin, but not CCP\u20102, were associated with an increased aortic plaque burden.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Citrullinated proteins are prevalent within atherosclerotic plaques, and certain ACPAs are associated with the atherosclerotic burden. These observations suggest that targeting of citrullinated epitopes, specifically Cit\u2010fibrinogen, within atherosclerotic plaques could provide a mechanism for the accelerated atherosclerosis observed in patients with RA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.37961","type":"journal-article","created":{"date-parts":[[2013,4,1]],"date-time":"2013-04-01T15:39:02Z","timestamp":1364830742000},"page":"1719-1724","source":"Crossref","is-referenced-by-count":133,"title":["Brief Report: Citrullination Within the Atherosclerotic Plaque: A Potential Target for the Anti\u2013Citrullinated Protein Antibody Response in Rheumatoid Arthritis"],"prefix":"10.1002","volume":"65","author":[{"given":"Jeremy","family":"Sokolove","sequence":"first","affiliation":[{"name":"Stanford University Medical Center, Stanford, California, and VA Palo Alto Health Care System Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matthew J.","family":"Brennan","sequence":"additional","affiliation":[{"name":"Stanford University Medical Center, Stanford, California, and VA Palo Alto Health Care System Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Orr","family":"Sharpe","sequence":"additional","affiliation":[{"name":"Stanford University Medical Center, Stanford, California, and VA Palo Alto Health Care System Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren J.","family":"Lahey","sequence":"additional","affiliation":[{"name":"Stanford University Medical Center, Stanford, California, and VA Palo Alto Health Care System Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amy H.","family":"Kao","sequence":"additional","affiliation":[{"name":"West Penn Allegheny Health System Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eswar","family":"Krishnan","sequence":"additional","affiliation":[{"name":"Stanford University Medical Center Stanford California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Edmundowicz","sequence":"additional","affiliation":[{"name":"Temple University School of Medicine Philadelphia Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christin M.","family":"Lepus","sequence":"additional","affiliation":[{"name":"Stanford University Medical Center, Stanford, California, and VA Palo Alto Health Care System Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary Chester","family":"Wasko","sequence":"additional","affiliation":[{"name":"West Penn Allegheny Health System Pittsburgh Pennsylvania"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William H.","family":"Robinson","sequence":"additional","affiliation":[{"name":"Stanford University Medical Center, Stanford, California, and VA Palo Alto Health Care System Palo Alto California"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,7,2]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30081"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0035296"},{"key":"e_1_2_7_4_1","first-page":"61","article-title":"Subclinical coronary artery calcification and relationship to disease duration in women with rheumatoid arthritis","volume":"35","author":"Kao AH","year":"2008","journal-title":"J Rheumatol"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2478"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1161\/01.ATV.19.9.2189"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1258\/acb.2007.007205"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23284"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2005.049403"},{"key":"e_1_2_7_10_1","doi-asserted-by":"crossref","first-page":"355","DOI":"10.1016\/S0308-2261(18)30021-3","article-title":"Fibrinogen, fibrin and fibrin degradation products in relation to atherosclerosis","volume":"15","author":"Smith EB","year":"1986","journal-title":"Clin Haematol"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200112)44:12<2737::AID-ART460>3.0.CO;2-#"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10419"},{"key":"e_1_2_7_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22482"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.37961","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.37961","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T03:53:39Z","timestamp":1696564419000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.37961"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7]]},"references-count":12,"journal-issue":{"issue":"7","published-print":{"date-parts":[[2013,7]]}},"alternative-id":["10.1002\/art.37961"],"URL":"https:\/\/doi.org\/10.1002\/art.37961","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7]]}}}