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Baxter Foundation (Career Development Award)"},{"name":"National Heart, Lung, and Blood Institute Proteomics","award":["contract HHSN288201000034C"],"award-info":[{"award-number":["contract HHSN288201000034C"]}]},{"DOI":"10.13039\/501100000024","name":"Canadian Institutes of Health Research","doi-asserted-by":"crossref","award":["2 OR-92141"],"award-info":[{"award-number":["2 OR-92141"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100007820","name":"Alliance for Lupus Research","doi-asserted-by":"crossref","award":["21858"],"award-info":[{"award-number":["21858"]}],"id":[{"id":"10.13039\/100007820","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Ben May Trust"},{"name":"Floren Family Trust"},{"DOI":"10.13039\/100011102","name":"European Union Seventh Framework Programme","doi-asserted-by":"crossref","award":["FP7\/2007-2013 grant 261382"],"award-info":[{"award-number":["FP7\/2007-2013 grant 261382"]}],"id":[{"id":"10.13039\/100011102","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Cure JM Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2013,9]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To evaluate serum interferon\u2010\u03b1 (IFN\u03b1) activity in the context of autoantibody profiles in patients with juvenile dermatomyositis (JDM).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sera from 36 patients with JDM were analyzed. Autoantibody profiles were determined by probing microarrays, which were fabricated with \u223c80 distinct autoantigens, with serum and a Cy3\u2010conjugated secondary antibody. Arrays were scanned and analyzed to determine antigen reactivity. Serum IFN\u03b1 activity was measured using a functional reporter cell assay. Sera were assayed alone or in combination with cellular material released from necrotic U937 cells to stimulate peripheral blood mononuclear cells from healthy donors in vitro, and IFN\u03b1 production in culture was measured by a dissociation\u2010enhanced lanthanide fluoroimmunoassay (DELFIA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Reactivity against at least 1 of 41 autoantigens on the microarray, including Ro 52, Ro 60, La, Sm, and RNP, was observed in 75% of the serum samples from patients with JDM. IFN\u03b1 activity was detected in 7 samples by reporter cell assay. The reporter cell assay showed a significant association of reactivity against Ro, La, Sm, and proliferating cell nuclear antigen with serum IFN\u03b1 activity (<jats:italic>P<\/jats:italic> = 0.005). Significance Analysis of Microarrays (SAM) identified increased reactivity against Sm, RNP, Ro 52, U1\u2010C, and Mi\u20102 in these sera. Sixteen samples induced IFN\u03b1 production as measured by DELFIA, and there was a significant association of reactivity against Ro, La, Sm, and RNP with the induction of IFN\u03b1 by serum and necrotic cell material (<jats:italic>P<\/jats:italic> = 0.034). SAM identified increased reactivity against Ro 60 in these sera.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These data support the hypothesis that nucleic acid\u2013associated autoantibodies, including the Ro\/La and Sm\/RNP complexes, may stimulate the production of active IFN\u03b1 in children with JDM.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.38038","type":"journal-article","created":{"date-parts":[[2013,6,5]],"date-time":"2013-06-05T16:12:32Z","timestamp":1370448752000},"page":"2424-2429","source":"Crossref","is-referenced-by-count":41,"title":["Brief Report: Interferon\u2010\u03b1 Induction and Detection of Anti\u2010Ro, Anti\u2010La, Anti\u2010Sm, and Anti\u2010RNP Autoantibodies by Autoantigen Microarray Analysis in Juvenile Dermatomyositis"],"prefix":"10.1002","volume":"65","author":[{"given":"Imelda","family":"Balboni","sequence":"first","affiliation":[{"name":"Stanford University Stanford California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy B.","family":"Niewold","sequence":"additional","affiliation":[{"name":"Mayo Clinic Rochester Minnesota"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gabrielle","family":"Morgan","sequence":"additional","affiliation":[{"name":"Ann and Robert H. Lurie Children's Research Center Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cindy","family":"Limb","sequence":"additional","affiliation":[{"name":"Stanford University Stanford California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maija\u2010Leena","family":"Eloranta","sequence":"additional","affiliation":[{"name":"Uppsala University and Uppsala University Hospital Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lars","family":"R\u00f6nnblom","sequence":"additional","affiliation":[{"name":"Uppsala University and Uppsala University Hospital Uppsala Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Paul J.","family":"Utz","sequence":"additional","affiliation":[{"name":"Stanford University Stanford California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lauren M.","family":"Pachman","sequence":"additional","affiliation":[{"name":"Ann and Robert H. Lurie Children's Hospital and Children's Research Center, and Northwestern University Feinberg School of Medicine Chicago Illinois"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,8,26]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(08)60955-1"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502132920706"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJM197502202920807"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780280208"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.168.8.4154"},{"key":"e_1_2_6_7_1","first-page":"S695","article-title":"Peripheral blood mononuclear cell gene expression profiles in children with juvenile dermatomyositis\/polymyositis (JDM\/PM) share type\u20101 interferon (IFN) signatures with systemic lupus erythematosus (SLE) but are distinct","volume":"54","author":"Pascual V","year":"2006","journal-title":"Arthritis Rheum"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.24555"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22860"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.24.021605.090709"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1038\/nm0302-295"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.10924"},{"key":"e_1_2_6_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11926-008-0053-2"},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.200800146"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gene.6364408"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.6.3519"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.091062498"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1080\/08916930400022681"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22164"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1542\/peds.2010-3022"}],"container-title":["Arthritis &amp; Rheumatism"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.38038","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.38038","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.38038","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,6]],"date-time":"2023-10-06T14:33:35Z","timestamp":1696602815000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1002\/art.38038"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,8,26]]},"references-count":19,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2013,9]]}},"alternative-id":["10.1002\/art.38038"],"URL":"https:\/\/doi.org\/10.1002\/art.38038","archive":["Portico"],"relation":{},"ISSN":["0004-3591","1529-0131"],"issn-type":[{"value":"0004-3591","type":"print"},{"value":"1529-0131","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,8,26]]}}}