{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,19]],"date-time":"2026-05-19T20:34:50Z","timestamp":1779222890419,"version":"3.51.4"},"reference-count":50,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,10,28]],"date-time":"2013-10-28T00:00:00Z","timestamp":1382918400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatism"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Complement component C4 copy number variations are associated with various inflammatory diseases. This study was undertaken to investigate whether copy number variations of C4 are also involved in the pathogenesis of Beh\u00e7et's disease (BD).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Gene expression was examined by enzyme\u2010linked immunosorbent assay (ELISA) or real\u2010time polymerase chain reaction (PCR). Copy number variations of C4 isotypes (C4A and C4B) were detected by real\u2010time PCR in 905 patients with BD, 205 patients with ankylosing spondylitis (AS) and acute anterior uveitis, and 1,238 controls. The activation of CD4+ T cells was analyzed by flow cytometry, and cytokine production was detected by ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Protein expression of total C4 in serum was significantly increased in patients with active BD compared with those with inactive BD or controls (Bonferroni corrected <jats:italic>P<\/jats:italic> [<jats:italic>P<\/jats:italic><jats:sub>corr<\/jats:sub>] = 1.64 \u00d7 10<jats:sup>\u22124<\/jats:sup> and <jats:italic>P<\/jats:italic><jats:sub>corr<\/jats:sub> = 0.037, respectively), but not in patients with AS and acute anterior uveitis. Copy number variation analysis identified a significantly increased frequency of more than 2 copies of C4A in BD patients (<jats:italic>P<\/jats:italic> = 1.65 \u00d7 10<jats:sup>\u22127<\/jats:sup>, odds ratio [OR] 2.84). HLA\u2013B51, which is located on the same chromosome as C4, showed a strong association with BD in the Han Chinese population (<jats:italic>P<\/jats:italic> = 8.90 \u00d7 10<jats:sup>\u221265<\/jats:sup>, OR 5.05), but logistic regression showed that C4A copy number variation was an independent risk factor for BD. A significantly increased expression of C4A was observed in the high copy number groups (&gt;2 copies or 2 copies) versus the low copy number group (<jats:italic>P<\/jats:italic><jats:sub>corr<\/jats:sub> = 0.019 and <jats:italic>P<\/jats:italic><jats:sub>corr<\/jats:sub> = 0.044, respectively). Increased production of interleukin\u20106 (IL\u20106) was also observed in the high C4A copy number group (<jats:italic>P<\/jats:italic><jats:sub>corr<\/jats:sub> = 0.037). No effect of C4 copy number variation on the expression of T cell activation markers was detected.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our findings indicate that a high copy number of C4A confers risk for BD by modulating the expression of C4A and enhancing IL\u20106 production.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.38116","type":"journal-article","created":{"date-parts":[[2013,8,5]],"date-time":"2013-08-05T20:32:49Z","timestamp":1375734769000},"page":"2963-2970","source":"Crossref","is-referenced-by-count":36,"title":["Copy Number Variations of Complement Component C4 Are Associated With Beh\u00e7et's Disease but Not With Ankylosing Spondylitis Associated With Acute Anterior Uveitis"],"prefix":"10.1002","volume":"65","author":[{"given":"Shengping","family":"Hou","sequence":"first","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, and Chongqing Key Laboratory of Ophthalmology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian","family":"Qi","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, and Chongqing Key Laboratory of Ophthalmology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dan","family":"Liao","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, and Chongqing Key Laboratory of Ophthalmology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi","family":"Zhang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, and Chongqing Key Laboratory of Ophthalmology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Fang","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, and Chongqing Key Laboratory of Ophthalmology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Zhou","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, and Chongqing Key Laboratory of Ophthalmology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunjia","family":"Liu","sequence":"additional","affiliation":[{"name":"The First Affiliated Hospital of Chongqing Medical University, Chongqing Eye Institute, and Chongqing Key Laboratory of Ophthalmology Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lin","family":"Bai","sequence":"additional","affiliation":[{"name":"The 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