{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,15]],"date-time":"2026-10-15T21:07:51Z","timestamp":1792098471627,"version":"4.4.0"},"reference-count":32,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2014,3,28]],"date-time":"2014-03-28T00:00:00Z","timestamp":1395964800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/3.0\/"}],"funder":[{"name":"Bioiberica, Spain"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2014,4]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To compare the gene expression patterns of synovial cells from inflamed or normal\/reactive areas of synovial membrane obtained from the same patient with osteoarthritis (OA).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>At the time of total knee replacement, synovial tissues were obtained from 12 patients with knee OA. The inflammation status of the synovial membrane was characterized according to macroscopic criteria and classified as normal\/reactive or inflamed. Biopsy samples were cultured separately for 7 days. Microarray gene expression profiling was performed on normal\/reactive and inflamed areas. Western blot and immunohistochemistry were used to confirm the identified genes that were differentially expressed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We identified 896 genes that were differentially expressed between normal\/reactive and inflamed areas. The key pathways were related to inflammation, cartilage metabolism, Wnt signaling, and angiogenesis. In the inflammation network, the genes TREM1 and S100A9 were strongly up\u2010regulated. The genes MMP3, MMP9, CTSH (cathepsin H), and CTSS (cathepsin S) were significantly up\u2010regulated in the cartilage catabolism pathway, while the most up\u2010regulated anabolism enzyme gene was HAS1. In the Wnt signaling pathway, the genes for Wnt\u20105a and low\u2010density lipoprotein receptor\u2013related protein 5 were up\u2010regulated, while the gene FZD2 and the gene for Dkk\u20103 were down\u2010regulated. Finally, STC1, which codes for a protein involved in angiogenesis, was identified as the most up\u2010regulated gene in inflamed compared with normal\/reactive areas.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>This study is the first to identify different expression patterns between 2 areas of the synovial membrane from the same patient. These differences concern several key pathways involved in OA pathogenesis. This analysis also provides information regarding new genes and proteins as potential targets of treatment.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.38315","type":"journal-article","created":{"date-parts":[[2013,12,24]],"date-time":"2013-12-24T08:11:11Z","timestamp":1387872671000},"page":"960-968","source":"Crossref","is-referenced-by-count":131,"title":["Gene Expression Pattern of Cells From Inflamed and Normal Areas of Osteoarthritis Synovial Membrane"],"prefix":"10.1002","volume":"66","author":[{"given":"C\u00e9cile","family":"Lambert","sequence":"first","affiliation":[{"name":"University of Li\u00e8ge and Centre Hospitalier Universitaire (CHU) Sart\u2010Tilman Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jean\u2010Emile","family":"Dubuc","sequence":"additional","affiliation":[{"name":"Cliniques Universitaires Saint\u2010Luc Brussels Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eul\u00e0lia","family":"Montell","sequence":"additional","affiliation":[{"name":"Bioiberica Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Josep","family":"Verg\u00e9s","sequence":"additional","affiliation":[{"name":"Bioiberica Barcelona Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carine","family":"Munaut","sequence":"additional","affiliation":[{"name":"University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Agn\u00e8s","family":"No\u00ebl","sequence":"additional","affiliation":[{"name":"University of Li\u00e8ge Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yves","family":"Henrotin","sequence":"additional","affiliation":[{"name":"University of Li\u00e8ge and Centre Hospitalier Universitaire (CHU) Sart\u2010Tilman Li\u00e8ge Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,3,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1089\/1549168041552964"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34453"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrrheum.2010.159"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1136\/ard.2004.025270"},{"key":"e_1_2_8_6_1","first-page":"2","article-title":"Is osteoarthritis a disease involving only cartilage or other articular tissues?","volume":"21","author":"Martel\u2010Pelletier J","year":"2010","journal-title":"Eklem Hastalik Cerrahisi"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bone.2012.02.012"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2005.01.005"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1053\/joca.2002.0829"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30137"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34315"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keh344"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1097\/BOR.0b013e3283103d12"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2007.01.020"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3771"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0950-3579(96)80045-8"},{"key":"e_1_2_8_17_1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/30.1.207"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1017\/erm.2013.4"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1136\/annrheumdis-2011-200281"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2009.01.009"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.164.10.4991"},{"key":"e_1_2_8_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imlet.2007.11.021"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2009.88"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10735-007-9117-2"},{"key":"e_1_2_8_25_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/kep452"},{"key":"e_1_2_8_26_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2003.08.004"},{"key":"e_1_2_8_27_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.11389"},{"key":"e_1_2_8_28_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2010.02.005"},{"key":"e_1_2_8_29_1","doi-asserted-by":"publisher","DOI":"10.1093\/rheumatology\/keq028"},{"key":"e_1_2_8_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2011.11.007"},{"key":"e_1_2_8_31_1","doi-asserted-by":"publisher","DOI":"10.1186\/1423-0127-18-39"},{"key":"e_1_2_8_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mce.2013.04.024"},{"key":"e_1_2_8_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.joca.2010.10.016"}],"container-title":["Arthritis &amp; Rheumatology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1002%2Fart.38315","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.38315","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1002\/art.38315","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/pdf\/10.1002\/art.38315","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T15:35:37Z","timestamp":1759937737000},"score":1,"resource":{"primary":{"URL":"https:\/\/acrjournals.onlinelibrary.wiley.com\/doi\/10.1002\/art.38315"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,3,28]]},"references-count":32,"journal-issue":{"issue":"4","published-print":{"date-parts":[[2014,4]]}},"alternative-id":["10.1002\/art.38315"],"URL":"https:\/\/doi.org\/10.1002\/art.38315","archive":["Portico"],"relation":{},"ISSN":["2326-5191","2326-5205"],"issn-type":[{"value":"2326-5191","type":"print"},{"value":"2326-5205","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,3,28]]}}}