{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:56:02Z","timestamp":1791064562316,"version":"4.1.0"},"reference-count":50,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,28]],"date-time":"2014-04-28T00:00:00Z","timestamp":1398643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Engagement of Fc\u03b3 receptor IIb (Fc\u03b3RIIb) suppresses B cell activation and represents a promising target for therapy in autoimmunity. The aim of this study was to characterize B cell immunosuppression mediated by the Fc\u2010engineered antibody, XmAb5871, which coengages Fc\u03b3RIIb with the B cell antigen receptor (BCR) complex and that is currently in clinical development for the treatment of rheumatoid arthritis (RA). Because rheumatoid factor (RF) might interfere with the binding of XmAb5871 to Fc\u03b3RIIb, we correlated RF titers with the potency of XmAb5871.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We analyzed the expression of CD19, Fc\u03b3RIIb, and CD86 on naive and memory B cells from 50 patients with RA and 66 healthy donors, quantified XmAb5871\u2010induced promotion of Fc\u03b3RIIb phosphorylation and suppression of calcium flux in activated B cells, measured CD86 inhibition in whole blood, and correlated RF and anti\u2212citrullinated protein antibody (ACPA) levels with drug potency. We engrafted RA peripheral blood mononuclear cells (PBMCs) into SCID mice, treated them with XmAb5871, and quantified human total IgG, total IgM, and anti\u2010tetanus IgG antibody levels in vivo.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>B cells from all donors expressed CD19 and Fc\u03b3RIIb, and the expression of Fc\u03b3RIIb was higher on naive, but not memory, B cells from donors with RA compared with healthy donors. BCR\u2010mediated calcium flux was suppressed by XmAb5871 and was associated with Fc\u03b3RIIb phosphorylation. XmAb5871 inhibited CD86 induction, and the levels of RF and ACPAs did not affect efficacy. XmAb5871 suppressed B cell activation regardless of disease severity. In SCID mice engrafted with PBMCs from a patient with RA, XmAb5871 suppressed humoral responses.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Coengagement of the BCR complex and Fc\u03b3RIIb by XmAb5871 inhibits B cell activation and function. The similar potency in patients with RA and healthy donors and the absence of autoantibody interference suggest that XmAb5871 may represent a new therapeutic strategy to suppress autoreactive B cells in RA.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.38334","type":"journal-article","created":{"date-parts":[[2013,12,24]],"date-time":"2013-12-24T08:19:12Z","timestamp":1387873152000},"page":"1153-1164","source":"Crossref","is-referenced-by-count":56,"title":["Suppression of Rheumatoid Arthritis B Cells by XmAb5871, an Anti\u2010CD19 Antibody That Coengages B Cell Antigen Receptor Complex and Fc\u03b3 Receptor IIb Inhibitory Receptor"],"prefix":"10.1002","volume":"66","author":[{"given":"Seung Y.","family":"Chu","sequence":"first","affiliation":[{"name":"Xencor, Inc. Monrovia California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Karen","family":"Yeter","sequence":"additional","affiliation":[{"name":"Los Angeles County + University of Southern California Medical Center and University of Southern California, Keck School of Medicine Los Angeles"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roshan","family":"Kotha","sequence":"additional","affiliation":[{"name":"Los Angeles County + University of Southern California Medical Center and University of Southern California, Keck School of Medicine Los Angeles"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erik","family":"Pong","sequence":"additional","affiliation":[{"name":"Xencor, Inc. Monrovia California"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yvonne","family":"Miranda","sequence":"additional","affiliation":[{"name":"Xencor, Inc. 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