{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T10:26:50Z","timestamp":1790332010653,"version":"4.1.0"},"reference-count":47,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2014,4,28]],"date-time":"2014-04-28T00:00:00Z","timestamp":1398643200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/3.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2014,5]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>Osteoarthritis (OA) is a common debilitating disease that results in degeneration of cartilage and bone in the synovial joints. Subtle changes in the molecular structure of the subchondral bone matrix occur and may be associated with cartilage changes. The aim of this study was to explore whether the abnormal molecular changes observed in the matrix of OA subchondral bone can be identified with Raman spectroscopy.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Tibial plateaus from patients undergoing total knee replacement for OA (n = 10) were compared with healthy joints from patients undergoing leg amputation (n = 5; sex\u2010 and laterality\u2010matched) and with non\u2010OA cadaveric knee specimens (n = 5; age\u2010matched). The samples were analyzed with Raman spectroscopy, peripheral quantitative computed tomography, and chemical analysis to compare changes in defined load\u2010bearing sites in both the medial and lateral compartments.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>OA subchondral bone matrix changes were detected by Raman spectroscopy. Within each cohort, there was no spectral difference in bone matrix chemistry between the medial and lateral compartments, whereas a significant spectral difference (<jats:italic>P<\/jats:italic> &lt; 0.001) was observed between the non\u2010OA and OA specimens. Type I collagen chain ratios were normal in the non\u2010OA specimens but were significantly elevated in the OA specimens.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>In comparing the results of Raman spectroscopy with those obtained by other standard techniques, these findings show, for the first time, that subchondral bone changes, or inherent differences, exist in both the medial and lateral (beneath intact cartilage) compartments of OA knees. The development of Raman spectroscopy as a screening tool, based on molecular\u2010specific modifications in bone, would facilitate the identification of clinical disease, including early molecular changes.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.38360","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T14:21:40Z","timestamp":1390832500000},"page":"1237-1246","source":"Crossref","is-referenced-by-count":38,"title":["Evidence from Raman Spectroscopy of a Putative Link Between Inherent Bone Matrix Chemistry and Degenerative Joint Disease"],"prefix":"10.1002","volume":"66","author":[{"given":"Jemma G.","family":"Kerns","sequence":"first","affiliation":[{"name":"Institute of Orthopaedics and Musculoskeletal Science, University College London, and Royal National Orthopaedic Hospital Stanmore UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Panagiotis D.","family":"Gikas","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedics and Musculoskeletal Science, University College London, and Royal National Orthopaedic Hospital Stanmore UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin","family":"Buckley","sequence":"additional","affiliation":[{"name":"Central Laser Facility, Science and Technology Facilities Council Rutherford Appleton Laboratory Didcot UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Adam","family":"Shepperd","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedics and Musculoskeletal Science, University College London, and Royal National Orthopaedic Hospital Stanmore UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen L.","family":"Birch","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedics and Musculoskeletal Science, University College London, and Royal National Orthopaedic Hospital Stanmore UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ian","family":"McCarthy","sequence":"additional","affiliation":[{"name":"Institute of Orthopaedics and Musculoskeletal Science, University College London, and Royal National Orthopaedic Hospital Stanmore UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jonathan","family":"Miles","sequence":"additional","affiliation":[{"name":"Royal National Orthopaedic Hospital Stanmore UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Timothy W. 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