{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T01:38:19Z","timestamp":1777426699183,"version":"3.51.4"},"reference-count":48,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2014,5,27]],"date-time":"2014-05-27T00:00:00Z","timestamp":1401148800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2014,6]]},"abstract":"<jats:sec><jats:title>Objective<\/jats:title><jats:p>To clarify the role of interleukin\u201020 (IL\u201020) in the regulatory mechanism of extracellular matrix expression and to determine the contribution of IL\u201020 to the phenotype of systemic sclerosis (SSc).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Protein and messenger RNA (mRNA) levels of collagen, Fli\u20101, IL\u201020, and IL\u201020 receptor (IL\u201020R) were analyzed using polymerase chain reaction (PCR) array, immunoblotting, immunohistochemical staining, enzyme\u2010linked immunosorbent assay, and real\u2010time PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>PCR array revealed that IL\u201020 decreased gene expression of \u03b12(I) collagen (0.03\u2010fold), Smad3 (0.02\u2010fold), and endoglin (0.05\u2010fold) in cultured normal dermal fibroblasts. Fli\u20101 protein expression was induced by IL\u201020 (\u223c2\u2010fold). The inhibition of collagen by IL\u201020, the induction of Fli\u20101 by IL\u201020, and the reduction of Smad3 and endoglin by IL\u201020 were also observed in SSc fibroblasts. Serum IL\u201020 levels were reduced only slightly in SSc patients but were significantly decreased in patients with scleroderma spectrum disorders (the prodromal stage of SSc) compared with those in normal subjects (111.3 pg\/ml versus 180.4 pg\/ml; <jats:italic>P<\/jats:italic> &lt; 0.05). On the other hand, IL\u201020 mRNA expression in SSc skin was decreased compared with that in normal skin (<jats:italic>P<\/jats:italic> &lt; 0.05), which may result in the induction of collagen synthesis in SSc dermal fibroblasts. IL\u201020R was expressed in normal and SSc fibroblasts. Moreover, IL\u201020 supplementation by injection into the skin reversed skin fibrosis induced by bleomycin in mice (\u223c0.5\u2010fold).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>IL\u201020 reduces basal collagen transcription via Fli\u20101 induction, while down\u2010regulation of Smad3 and endoglin may cancel the effect of transforming growth factor \u03b2 in SSc fibroblasts. To confirm the therapeutic value of IL\u201020 and IL\u201020R, their function and expression in vivo should be further studied.<\/jats:p><\/jats:sec>","DOI":"10.1002\/art.38380","type":"journal-article","created":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T14:26:29Z","timestamp":1390832789000},"page":"1636-1647","source":"Crossref","is-referenced-by-count":14,"title":["Decreased Interleukin\u201020 Expression in Scleroderma Skin Contributes to Cutaneous Fibrosis"],"prefix":"10.1002","volume":"66","author":[{"given":"Hideo","family":"Kudo","sequence":"first","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Masatoshi","family":"Jinnin","sequence":"additional","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yoshihide","family":"Asano","sequence":"additional","affiliation":[{"name":"University of Tokyo Graduate School of Medicine Tokyo Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Trojanowska","sequence":"additional","affiliation":[{"name":"Boston University School of Medicine Boston, Massachusetts"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wakana","family":"Nakayama","sequence":"additional","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kuniko","family":"Inoue","sequence":"additional","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Noritoshi","family":"Honda","sequence":"additional","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ikko","family":"Kajihara","sequence":"additional","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katsunari","family":"Makino","sequence":"additional","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satoshi","family":"Fukushima","sequence":"additional","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hironobu","family":"Ihn","sequence":"additional","affiliation":[{"name":"Kumamoto University Kumamoto Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,5,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0923-1811(00)00143-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1007\/s001090050216"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI31139"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra0806188"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1002\/1529-0131(200102)44:2<474::AID-ANR67>3.0.CO;2-#"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI16269"},{"key":"e_1_2_6_8_1","first-page":"755","article-title":"Hypothesis: pathogenesis of systemic sclerosis","volume":"30","author":"Takehara K","year":"2003","journal-title":"J Rheumatol"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar3315"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.30647"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1994.tb06108.x"},{"key":"e_1_2_6_12_1","first-page":"1207","article-title":"Mechanisms of pathogenesis in scleroderma. 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