{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,8]],"date-time":"2026-09-08T12:26:08Z","timestamp":1788870368999,"version":"build-2803163510"},"reference-count":46,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T00:00:00Z","timestamp":1406505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000738","name":"U.S. Department of Veterans Affairs","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000738","id-type":"DOI","asserted-by":"publisher"}]},{"name":"The Frederick G.L. Huetwell and William D. Robinson, MD Professorship in Rheumatology"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>To determine the role of \u03b1(1,2)\u2010linked fucosylation of proteins by fucosyltransferase 1 (FUT1) in rheumatoid arthritis (RA) angiogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Analysis of \u03b1(1,2)\u2010linked fucosylated proteins in synovial tissue (ST) samples was performed by immunohistologic staining. Expression of \u03b1(1,2)\u2010linked fucosylated angiogenic chemokine in synovial fluid (SF) was determined by immunoprecipitation and lectin blotting. To determine the angiogenic role of \u03b1(1,2)\u2010linked fucosylated proteins in RA, we performed human dermal microvascular endothelial cell (HMVEC) chemotaxis and Matrigel assays using sham\u2010depleted and \u03b1(1,2)\u2010linked fucosylated protein\u2013depleted RA SF samples. To examine the production of proangiogenic chemokines by FUT1 in HMVECs, cells were transfected with FUT1 sense or antisense oligonucleotides, and enzyme\u2010linked immunosorbent assay was performed. We then studied mouse lung endothelial cell (EC) chemotaxis using wild\u2010type and FUT1 gene\u2013deficient mouse lung ECs.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>RA ST endothelial cells showed high expression of \u03b1(1,2)\u2010linked fucosylated proteins compared to normal ST. The expression of \u03b1(1,2)\u2010linked fucosylated monocyte chemoattractant protein 1 (MCP\u20101)\/CCL2 was significantly elevated in RA SF compared with osteoarthritis SF. Depletion of \u03b1(1,2)\u2010linked fucosylated proteins in RA SF induced less HMVEC migration and tube formation than occurred in sham\u2010depleted RA SF. We found that blocking FUT1 expression in ECs resulted in decreased MCP\u20101\/CCL2 and RANTES\/CCL5 production. Finally, we showed that FUT1 regulates EC migration in response to vascular endothelial cell growth factor.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>Our findings indicate that \u03b1(1,2)\u2010linked fucosylation by FUT1 may be an important new target for angiogenic diseases such as RA.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.38648","type":"journal-article","created":{"date-parts":[[2014,4,1]],"date-time":"2014-04-01T14:09:50Z","timestamp":1396361390000},"page":"2047-2058","source":"Crossref","is-referenced-by-count":15,"title":["Fucosyltransferase 1 Mediates Angiogenesis in Rheumatoid Arthritis"],"prefix":"10.1002","volume":"66","author":[{"given":"Takeo","family":"Isozaki","sequence":"first","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad A.","family":"Amin","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey H.","family":"Ruth","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Phillip L.","family":"Campbell","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pei\u2010Suen","family":"Tsou","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christine M.","family":"Ha","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"W. 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