{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T23:35:49Z","timestamp":1791329749711,"version":"4.3.1"},"reference-count":23,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2014,7,28]],"date-time":"2014-07-28T00:00:00Z","timestamp":1406505600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"French Society of Rheumatology"},{"name":"Passerelle Grant (pfizer)"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Regulatory interleukin\u201010 (IL\u201010)\u2013producing B cells (B10 cells) have been shown to prevent and cure collagen\u2010induced arthritis in mice. In humans, very little is known about B10 cells in rheumatoid arthritis (RA). Several B cell subsets, such as CD24\n                      <jats:sup>high<\/jats:sup>\n                      CD38\n                      <jats:sup>high<\/jats:sup>\n                      , CD24\n                      <jats:sup>high<\/jats:sup>\n                      CD27+, and CD5+ B cells, were suggested to be precursors of B10 cells. We aimed to analyze these B cell subsets and B10 cells in RA patients and healthy controls.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>B10 cells were generated from peripheral blood mononuclear cells stimulated for 24 hours with CpG and for 4 hours with phorbol 12\u2010myristate 13\u2010acetate\/ionomycin\/brefeldin A. Intracellular B cell IL\u201010 was assessed by flow cytometry. Thirty\u2010one controls and 99 RA patients were included.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      After multiple adjustments, levels of CD24\n                      <jats:sup>high<\/jats:sup>\n                      CD38\n                      <jats:sup>high<\/jats:sup>\n                      , CD24\n                      <jats:sup>high<\/jats:sup>\n                      CD27+, and CD5+ B cells were found to be similar in RA patients and controls. Levels of B10 cells were lower in RA patients than in controls, especially in patients with RA of \u22645 years' duration. Levels of B10 cells correlated inversely with the Disease Activity Score in 28 joints. This was more pronounced in patients with RA of \u22645 years' duration, in whom B10 cells also correlated inversely with C\u2010reactive protein levels. Moreover, B10 cells correlated inversely with rheumatoid factor levels. CD24\n                      <jats:sup>high<\/jats:sup>\n                      CD38\n                      <jats:sup>high<\/jats:sup>\n                      and CD24\n                      <jats:sup>high<\/jats:sup>\n                      CD27+ B cells induced more Treg cells than did CD24\n                      <jats:sup>low<\/jats:sup>\n                      B cells in controls but not in RA patients.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The ability of B cells to produce IL\u201010 was altered in RA, and this impairment influenced disease activity, biologic inflammation, and autoantibody levels, especially in patients with RA of \u22645 years' duration. This strongly suggests a role of B10 cells in RA initiation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.38666","type":"journal-article","created":{"date-parts":[[2014,4,11]],"date-time":"2014-04-11T11:30:59Z","timestamp":1397215859000},"page":"2037-2046","source":"Crossref","is-referenced-by-count":144,"title":["Regulatory B10 Cells Are Decreased in Patients With Rheumatoid Arthritis and Are Inversely Correlated With Disease Activity"],"prefix":"10.1002","volume":"66","author":[{"given":"Claire I.","family":"Daien","sequence":"first","affiliation":[{"name":"Lapeyronie Hospital, Montpellier 1 University, and Institute of Molecular Genetics of Montpellier, UMR5535, CNRS Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah","family":"Gailhac","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics of Montpellier, UMR5535, CNRS Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thibault","family":"Mura","sequence":"additional","affiliation":[{"name":"St. Eloi Hospital Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rachel","family":"Audo","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics of Montpellier, UMR5535, CNRS Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bernard","family":"Combe","sequence":"additional","affiliation":[{"name":"Lapeyronie Hospital, Montpellier 1 University, and Institute of Molecular Genetics of Montpellier, UMR5535, CNRS Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Hahne","sequence":"additional","affiliation":[{"name":"Institute of Molecular Genetics of Montpellier, UMR5535, CNRS Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jacques","family":"Morel","sequence":"additional","affiliation":[{"name":"Lapeyronie Hospital, Montpellier 1 University, and Institute of Molecular Genetics of Montpellier, UMR5535, CNRS Montpellier France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,7,28]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.23176"},{"key":"e_1_2_8_3_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI25422"},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.12.7868"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2012.03.010"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2008.03.017"},{"key":"e_1_2_8_7_1","doi-asserted-by":"publisher","DOI":"10.2119\/molmed.2011.00333"},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.11.009"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2002-05-1525"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.27584"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.1780380107"},{"key":"e_1_2_8_12_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2010-07-294249"},{"key":"e_1_2_8_13_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.34364"},{"key":"e_1_2_8_14_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0900270"},{"key":"e_1_2_8_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201040673"},{"key":"e_1_2_8_16_1","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.3005407"},{"issue":"39","key":"e_1_2_8_17_1","first-page":"S100","article-title":"The Simplified Disease Activity Index (SDAI) and the Clinical Disease Activity Index (CDAI): a review of their usefulness and validity in rheumatoid arthritis","volume":"23","author":"Aletaha D","year":"2005","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_8_18_1","doi-asserted-by":"publisher","DOI":"10.1186\/ar2718"},{"key":"e_1_2_8_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jaut.2007.07.002"},{"key":"e_1_2_8_20_1","doi-asserted-by":"publisher","DOI":"10.1002\/art.22279"},{"key":"e_1_2_8_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-3083.1992.tb02846.x"},{"key":"e_1_2_8_22_1","first-page":"201","article-title":"Broadening of the T cell receptor spectrum among rheumatoid arthritis synovial cell\u2010lines in relation to disease duration","volume":"18","author":"Elewaut D","year":"2000","journal-title":"Clin Exp Rheumatol"},{"key":"e_1_2_8_23_1","doi-asserted-by":"publisher","DOI":"10.3899\/jrheum.100324"},{"key":"e_1_2_8_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10067-013-2359-3"}],"container-title":["Arthritis &amp; 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