{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T19:03:19Z","timestamp":1792782199034,"version":"4.5.0"},"reference-count":39,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2014,12,27]],"date-time":"2014-12-27T00:00:00Z","timestamp":1419638400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"NIH\/NIAMS","award":["AR38477"],"award-info":[{"award-number":["AR38477"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Arthritis &amp; Rheumatology"],"published-print":{"date-parts":[[2015,1]]},"abstract":"<jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>Aminopeptidase N\/CD13 (EC 3.4.11.2) is a metalloproteinase expressed by fibroblast\u2010like synoviocytes (FLS). It has been suggested that CD13 can act chemotactically for T cells in rheumatoid arthritis (RA). We undertook this study to measure CD13 in vivo and in vitro in RA samples and to determine whether CD13 could play a role in the homing of T cells to the RA joint.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>Interleukin\u201017\u2013treated FLS were used to immunize mice, from which a novel anti\u2010human CD13 monoclonal antibody (mAb), 591.1D7.34, was developed. The mAb 591.1D7.34 and a second anti\u2010CD13 mAb, WM15, were used to develop a novel enzyme\u2010linked immunosorbent assay (ELISA) for CD13, and CD13 enzymatic activity was measured in parallel. Chemotaxis of cytokine\u2010activated T cells was measured by a chemotaxis\u2010under\u2010agarose assay.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>We detected substantial amounts of CD13 in synovial fluid (SF), sera, FLS lysates, and culture supernatants by ELISA, with a significant increase in CD13 in RA SF when compared to osteoarthritis SF. CD13 accounted for most but not all of the CD13\u2010like enzymatic activity in SF. Recombinant human CD13 was chemotactic for cytokine\u2010activated T cells through a G protein\u2013coupled receptor and contributed to the chemotactic properties of SF independently of enzymatic activity.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>CD13 is released from FLS into culture supernatants and is found in SF. CD13 induces chemotaxis of cytokine\u2010activated T cells, a T cell population similar to that found in RA synovium. These data suggest that CD13 could play an important role as a T cell chemoattractant, in a positive feedback loop that contributes to RA synovitis.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1002\/art.38878","type":"journal-article","created":{"date-parts":[[2014,9,12]],"date-time":"2014-09-12T14:47:35Z","timestamp":1410533255000},"page":"74-85","source":"Crossref","is-referenced-by-count":48,"title":["Expression and Function of Aminopeptidase N\/CD13 Produced by Fibroblast\u2010like Synoviocytes in Rheumatoid Arthritis: Role of CD13 in Chemotaxis of Cytokine\u2010Activated T Cells Independent of Enzymatic Activity"],"prefix":"10.1002","volume":"67","author":[{"given":"Rachel","family":"Morgan","sequence":"first","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Judith","family":"Endres","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nilofar","family":"Behbahani\u2010Nejad","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kristine","family":"Phillips","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jeffrey H.","family":"Ruth","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sean C.","family":"Friday","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gautam","family":"Edhayan","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thomas","family":"Lanigan","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Urquhart","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kevin C.","family":"Chung","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David A.","family":"Fox","sequence":"additional","affiliation":[{"name":"University of Michigan Ann Arbor"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,12,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI114015"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0167-5699(98)01398-X"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.2174\/092986707780059571"},{"key":"e_1_2_6_5_1","first-page":"361","article-title":"The moonlighting enzyme CD13: old and new functions to target","volume":"14","author":"Mina\u2010Osorio P","year":"2008","journal-title":"Cell"},{"key":"e_1_2_6_6_1","first-page":"1695","article-title":"CD13 (GP150; 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